Waldo Medina

7 Questions 11 Answers 0 Followers

Questions related from Waldo Medina

Could a coating antibody also be used as detection antibody in an ELISA sandwich assay?

I want to make a sandwich ELISA for the detection of antigen X. I want to know if I can use the same antibody as detection and coating (detection antibody will be conjugated to HRP).

11 November 2015 1,445 7 View

What preservative should be used for Ag storage in an indirect ELISA?

I want to make an indirect ELISA (Ag+anti-Ag+anti-anti-agHRP). And I want to reconstitute the lyophilized antigen (peptides). The question is, what preservative should be used for Ag storage , if...

08 August 2015 9,737 3 View

What is the best method for obtaining derived-vaccine antigen to be used in in vitro T cell proliferation assays?

I want to make an assay of in vitro proliferation of PBMC in animals immunized using the same vaccine antigen. To do this I have to process the vaccine. Among the existing techniques as UV,...

10 October 2014 2,448 5 View

Where can I buy LSCC-RP9 cell line?

I need the cell line for determining NK cell cytototoxicity in chickens. I have not found a commercial house that sells it.

08 August 2014 9,614 0 View

Does anyone know of any protocol to obtain an ethanolic extract of Euphorbia plants latex?

I want to make a study using the ethanolic extract of a Euphorbia latex on tumor cells in culture. I have tried some protocols, but do not get a good result, since the formation of precipitates in...

06 June 2014 1,193 3 View

What is the lifetime of a flow cytometer laser?

03 March 2014 5,484 3 View

Is it possible to fix PBMCs and after one or two weeks add the antibodies (CD4/CD8) for immunophenotyping flow cytometry?

I need to urgently analyze some samples this week, but I have not yet antibodies. They take 2 or 3 weeks to arrive, so I want to know if I can keep the fixed cells. Will there be any change?

10 October 2013 4,360 4 View