I want to know the influence of different diafiltration mediums to know on the retentate and permeate obtained. I want to know which types exist and which effect produce. Thank you for your help.
Hello Manoella Ajcet. The normal diafiltration medium used in most of the DF processes is pure water. However in some cases in which you need that the components of your diafiltration retentate are chemically stable (e.g., enzymes, proteins, or microorganisms) you may need to try different diafiltration media. In such cases you need to use buffers or saline solutions. In general, the minerals used for buffer preparation should not affect importantly the flux of your permeate, nor the rejection coefficients of the main macromolecules.
Hope this answers your question. Regards.
Thank you so much Rogelio Valadez-Blanco. I was in doubt about that, cause I didn't found too many papers talking about de DF media. Thank you!
For selected dairy application you may also consider using permeates coming from your own separation processes. Properly selected permeates have no fouling properties and do not dilute your feed (in terms of lactose and mineral salts) while helping to decrase the content of proteins thus enhancing their separation.
An interesting application of using UF permeate as a DF media you may find in: A Microfiltration Process to Maximize Removal of Serum Proteins from Skim Milk Before Cheese Making by B. K. Nelson and D. M. Barbano
- Badges
- Science topic
- Similar topics
- Biological Science
- Biochemistry
- Biochemical Techniques
- Analytical Chemistry
- pH