Shangjian Luo

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Questions related from Shangjian Luo

When inducing pyroptosis, is it necessary to remove LPS and then add ATP?

In the experiment of inducing pyroptosis of THP1 cells, when adding ATP after pretreatment of cells with LPS, is it necessary to remove the LPS and then add the medium containing ATP, or add ATP...

08 May 2023 1,818 1 View

When infecting Caco-2 cells with lentivirus, what is the final concentration of polybrene in the dish?

How much polybrene should be added when infecting Caco-2 cells with lentivirus?

23 August 2022 3,230 1 View

The apoptosis of Caco-2 detected by flow cytometry , the negative control cells die too much,is there any solution?

When the apoptosis of Caco-2 was detected by flow cytometry , the negative control cells die too much, especially the proportion of cells in the upper left quadrant is too large. is there any...

22 June 2022 4,568 0 View

How to induce pyroptosis in THP1 cell?

In the experiment of inducing pyroptosis of THP1 cells, when adding ATP after pretreatment of cells with LPS, is it necessary to remove the LPS and then add the medium containing ATP, or add ATP...

01 January 1970 3,282 1 View