As i culture my cells, i noticed that some of them got bigger and round.The background is clear, and the cell growth rate is fine.Can someone please tell me why the cell morphology changes?
Hello Liu Xiaoping
It is a sign that those cells are undergoing mitosis. In adherent cell culture, this process begins in early prophase when cells decrease substrate adhesion and round up to assume a characteristic spherical shape. This process of cell rounding in early mitosis is a feature in animal cell division which is observed in cells in 2D and 3D culture. By creating a spherical cell shape at metaphase, mitotic rounding ensures that there is sufficient space within the cell to form mitotic spindle. So, you will observe a change in morphology in these cells.
I would be glad if you could post a pic of the culture showing those rounded bigger cells.
Best.
Good afternoon, Liu Xiaoping,
Spherical cells cannot be mitotic? An increase in cell size may be a consequence of spontaneous polyploidization. Which, in turn, is the result of incomplete cytotomy, that is, separation of the cytoplasm after mitosis. Such incomplete cytotomy is typical for cells that are well attached to the culture substrate. Do "large cells" have one nucleus or two? Can you follow the progression of mitosis in large cells by vital observation? If they are polyploid, then there is a high probability that the mitoses in them will be tripolar or quadripolar. This can be visible in fixed cells too.
Best regards,
Rustem Uzbekov
tks for your reply,the picture post below contains some rounded bigger.According to the shape of it's cell nucleus ,i don't think it's undergoing
mitosis.@Malcolm Nobre
tks for your reply.When detaching cell from plate using tripsin,i usually put it in incubator for 1-2 min.Maybe it's because the cell detachment time is to short?@Rustem E Uzbekov
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