We are considering a move from C2C12 cells to HSKM cells since the former is not applicable for GLUT4 studies, however we don't have any experience with human cell lines. My questions are:

1. What is the max. passage possible on HSKM cells?

2. Can they be grown / split / differentiated similarly to C2C12?

3. Are there any issues regarding their expression of GLUT4 or Insulin Receptor? 

Thank you in advance, 

Stefan

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