Stefan Kühn

2 Questions 5 Answers 0 Followers

Questions related from Stefan Kühn

What's the max. passage on HSKM cells / can they be grown/split/differentiated similarly to C2C12 cells?

We are considering a move from C2C12 cells to HSKM cells since the former is not applicable for GLUT4 studies, however we don't have any experience with human cell lines. My questions are:1. What...

14 May 2016 4,394 1 View

How can I best determine extracellular (media) glucose concentration of differentiated C2C12 cells (non-radiometric)?

I'm trying to measure the extracellular glucose of my cells to get a feel for their rate of glucose uptake (from a fixed concentration of glucose), however I'm not really an experimentalist and am...

11 January 2016 9,541 3 View