What's the in vitro method I could apply to validate the function of certain histone modification?

04 December 2015 1 3K Report

Hi all,

I found some interesting histone modification markers that might contribute to the "open" or "closed" state of the expression of a gene by meta data analysis. I wonder if there's way to validate its function in a cell model? I know to validate a transcriptional factor we can do a transfection assay and reporter gene assay, but when it comes to histone modification, it is different and I don't have such experience.

Many thanks!

Xiaojing Tang

Hi Alice,

Thanks for your answer. Actually I got the correlation from CHIP-seq...Now I want to know how I can do any functional test....

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