SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

How can I can quantify the protein in sample with lammeli buffer?

I used lammeli buffer to direct extract of bacterial protein for SDS Page, how can I quantify the conc of the protein in the sample with Bradford assay as its the only method available to me

18 January 2021 9,581 5 View

I am asking about the drug release from minicells, to test the antimicrobial activity of the drug?

I am working on minicells loaded with the drug, I want to test the antimicrobial activity of the drug loaded in minicells using agar diffusion method, many trials didn't produce any zone of...

17 January 2021 7,511 3 View

I am looking for this strain, Lactobacillus acidophilus (strain for minicells production). Please can you help me to find it?

I am a master student, I need this strain for research purposes. Lactobacillus acidophilus [ minicells producing strain (Minicells are generally produced by aberrant cell divisions at...

13 March 2020 3,204 3 View

Minicells loading and release of Drug

Minicells can be used as drug carrier, the loading of antibiotics was performed by incubation with rotation and the release was done by extraction of drug. Is there are any other methods for...

01 January 1970 8,747 1 View

How to select substrate concentration to determine the inhibitor behavior as competitive or non-competitive?

I already calculated the IC50 value of the inhibitor against the protease enzyme. Now I need to find this inhibitor competitive non-competitive. I decided to perform the Lineweaver Burk plot, but...

21 January 2021 8,378 3 View

Best purification method for recombinant protein in BL21?

Hi I need to purify a recombinant protein (IL6 21kDa). It's been produced in E.coli BL21 with a T7 expression vector, but it does not have a tag as yet. I was thinking of adding a his-tag and...

09 December 2020 2,200 8 View

How to handle milk clotting Issues during media preparation for Screening of Halotolerant Protease Producing Bacteria (with Skim Milk)?

We want to screen halotolerant protease producing bacteria. Since Skim milk (HiMedia) is available in our lab, therefore, we want to screen extracellular protease by this compound/ supplement. We...

04 December 2020 7,657 6 View

Can TEV protease cleave between glutamine and methionine efficiently?

Based on this paper The P1 ' specificity of tobacco etch virus protease ,TEV protease can cleave between the Gln and several amino-acids (besides Gly/Ser) with acceptable efficiency in its...

26 November 2020 5,987 4 View

What is the best method to check interaction between a drug and a protein ?

Hi, I would like to know the best method to check the interaction between a proteine (cruzipain, cysteine protease) and a drug. It has been purposed to our project to evaluate protein-drug...

30 October 2020 7,209 3 View

How to purify a recombinant protein from Ecoli to reach a cell culture purpose?

05 October 2020 502 7 View

Protease activity assay?

Hello, I would like to get help! I want to measure the protease activity of pancrease powder. I prepared 10mg/ml stock solution from the pancrease powder and I used 100ul from that and I added...

22 June 2020 1,609 3 View

Why my protein has lower molecular weight that expected?

Hi, I am trying to make a kind of recombinant protein (Flag-tagged) from HEK293T cells. But when I check the cell lysate by WB, the molecular weight is lower that expected. The molecular weight...

09 June 2020 6,414 13 View

No protein expression in Pichia, any ideas?

I introduced a heterologous gene using pPIC 9 plasmid into KM71 strain and confirmed the presence of the target gene by PCR. The colonies (6) I am checking growing well on Minimal growth media...

25 May 2020 1,611 9 View

In which environments would you bioprospect microorganisms with proteolytic activity?

I need to isolate microorganisms that can produce proteases, in which environments can I find them Justify answer Thank you

27 April 2020 7,669 1 View