What's the concentration of protease produced by P.aeruginosa and K.pneumonia?

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SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,814 3 View

How can I can quantify the protein in sample with lammeli buffer?

I used lammeli buffer to direct extract of bacterial protein for SDS Page, how can I quantify the conc of the protein in the sample with Bradford assay as its the only method available to me

18 January 2021 9,801 5 View

I am asking about the drug release from minicells, to test the antimicrobial activity of the drug?

I am working on minicells loaded with the drug, I want to test the antimicrobial activity of the drug loaded in minicells using agar diffusion method, many trials didn't produce any zone of...

17 January 2021 7,738 3 View

I am looking for this strain, Lactobacillus acidophilus (strain for minicells production). Please can you help me to find it?

I am a master student, I need this strain for research purposes. Lactobacillus acidophilus [ minicells producing strain (Minicells are generally produced by aberrant cell divisions at...

13 March 2020 3,419 3 View

Minicells loading and release of Drug

Minicells can be used as drug carrier, the loading of antibiotics was performed by incubation with rotation and the release was done by extraction of drug. Is there are any other methods for...

01 January 1970 8,975 1 View

Can it is possible to find the cleaved sequence when a protein cleaved by a heamaglutanin protease (HA/P) by any bioinformatics tools?

Bioinformatics tools like peptide cutter

15 July 2024 6,453 1 View

Does the catalytic activity of a lyase, such as a protease, change upon the formation of a μ-gelzyme?

21 May 2024 9,403 0 View

Why are protease inhibitors necessary in denaturing conditions?

I know that conventional wisdom dictates that protease inhibitors should be added to lysis buffers when extracting protein to prevent sample degradation. That said, why are such inhibitors...

14 May 2024 2,145 2 View

What tool can predict E. coli protease cleavage sites?

I am observing cleavage in my construct expressed in E. coli and attempting to identify the responsible proteases. Mass spectrometry analysis has revealed the sizes of the fragments, enabling...

12 May 2024 6,540 2 View

How to prepare a perfusion buffer and calculate the concentrations of each enzyme?

I want to prepare perfusion buffer consisting of: collagenase B (2.5 mg/ml), Dispase II (1.2 U/ml; Roche), Bacillus licheniformis protease 7.5 mg/ml (P5380; Sigma), DNase I (LS002058, 125...

02 May 2024 4,928 0 View

My recombinant enzyme activity is acting weird, does anyone have an explanation, or even know how to solve this problem?

I'm working with recombinant protein, it's a protease enzyme that we use E. coli to synthesize, and usually, it's stable for up to six months in the -80 freezer. Recently, the protein activity has...

17 April 2024 4,262 3 View

How can I develop an enzyme assay for degradation of gluten( low quantity of enzyme available)?

I need to develop an enzyme assay to evaluate the degradation of gluten using proteases. Previously, I conducted tests with finished products, which was straightforward. However, I now have to...

07 April 2024 1,886 3 View

What do I do if my Bradford Reagent yields underestimated protein concentration for measuring trypsin?

I use 0.25% trypsin (2.5g/L) as a positive control in my protease assays. I'm trying to measure the stability of different proteases overtime and at different temps. When I measure the...

30 March 2024 8,491 0 View

Troubleshooting SUMOylation Detection in A. evenia and Tobacco Plant total protein Extracts?

I am currently conducting a Western blot analysis on A. evenia and tobacco (Nb) plant total protein extracts expressing SUMO protein in order to detect SUMOylation using an anti-SUMO antibody....

25 March 2024 5,825 0 View

What is the most effective enzyme for breaking down gluten formed when flour is mixed with water?

I've selected protease as the optimal enzyme for eliminating gluten formed from flour. Could you please provide insights on the best enzyme for removing gluten, dosing methods, and how to identify...

24 March 2024 1,618 2 View