Does the catalytic activity of a lyase, such as a protease, change upon the formation of a μ-gelzyme?
I’m trying to reproduce the acousto-optic coupling simulation in the paper Frequency–angular resolving LiDAR using chip-scale acousto-optic beam steering. LNOI(lithium niobate on insulator) is...
13 August 2024 9,793 0 View
Request Python code from this article : Gender equity of authorship in pulmonary medicine over the past decade. THANKS!
08 August 2024 6,241 2 View
One of my friends, every night after falling asleep, his mouth would make a creaking sound after chewing something. This teeth grinding condition would continue until he woke up, and he didn't...
08 August 2024 1,366 5 View
It has been documented that apoptotic cells themselves can induce phosphorylation of serine 139 on H2AX (γH2AX) due to DNA fragmentation during apoptosis (doi: 10.1074/jbc.275.13.9390). As γH2AX...
28 July 2024 7,982 2 View
Hi there, I'm new to modelling with comsol. I would like to ask if it is possible for comsol to output linearly polarized (LP) modes? I tried modelling a simple single mode fiber with an enlarged...
23 July 2024 4,018 3 View
What is the future scope of acoustic emission?
17 July 2024 1,508 1 View
I read two papers which uses Instantaneous SINR (ISINR) as criterion for performance evaluation. In the two papers below, it seems the desired signal (numerator) in ISINR does not involve...
16 July 2024 4,545 0 View
Hi, I'm analyzing some bulk RNAseq data from T cells under different conditions, and based on the RNAseq data, condition A shows higher expression of genes associated with effector functions...
01 July 2024 740 2 View
I want to extract basement shear force in LS-DYNA calculation result, but i found that there were no force results but only moment results in SPCFORC even I check the model and make sure the...
23 June 2024 2,691 0 View
I am evaluating the apoptosis of two types of cells, which are derived from primary mammalian cells (fibroblasts). When I use the Annexin V/PI method, the image I get is like this. It seems not...
17 June 2024 5,569 1 View
Bioinformatics tools like peptide cutter
15 July 2024 6,453 1 View
I know that conventional wisdom dictates that protease inhibitors should be added to lysis buffers when extracting protein to prevent sample degradation. That said, why are such inhibitors...
14 May 2024 2,145 2 View
I am observing cleavage in my construct expressed in E. coli and attempting to identify the responsible proteases. Mass spectrometry analysis has revealed the sizes of the fragments, enabling...
12 May 2024 6,540 2 View
I want to prepare perfusion buffer consisting of: collagenase B (2.5 mg/ml), Dispase II (1.2 U/ml; Roche), Bacillus licheniformis protease 7.5 mg/ml (P5380; Sigma), DNase I (LS002058, 125...
02 May 2024 4,927 0 View
I'm working with recombinant protein, it's a protease enzyme that we use E. coli to synthesize, and usually, it's stable for up to six months in the -80 freezer. Recently, the protein activity has...
17 April 2024 4,261 3 View
I need to develop an enzyme assay to evaluate the degradation of gluten using proteases. Previously, I conducted tests with finished products, which was straightforward. However, I now have to...
07 April 2024 1,886 3 View
I use 0.25% trypsin (2.5g/L) as a positive control in my protease assays. I'm trying to measure the stability of different proteases overtime and at different temps. When I measure the...
30 March 2024 8,491 0 View
I am currently conducting a Western blot analysis on A. evenia and tobacco (Nb) plant total protein extracts expressing SUMO protein in order to detect SUMOylation using an anti-SUMO antibody....
25 March 2024 5,824 0 View
I've selected protease as the optimal enzyme for eliminating gluten formed from flour. Could you please provide insights on the best enzyme for removing gluten, dosing methods, and how to identify...
24 March 2024 1,618 2 View
Accurate way to determine the cleavage site of the protein if the cleavage can not be determined by Mass Spectroscopy?
15 March 2024 5,225 1 View