I need to make a 10 mM Tris solution, pH 7.4, that is RNAse free. I have RNAse-free Tris and RNAse free water to make the 10 mM Tris solution. How's the best way logistically to weigh out the amount needed (~12 mg) on an analytical balance in a way that doesn't contaminate the solution with RNAse. Typically I would use an autoclaved spatula and weigh paper, but I'm pretty sure neither of those in our lab are RNAse free. Any tips would be welcome!
Do you have RNAseZap or RNAse Away spray? They're different types of sprays but I pretty much spray everything (pippettes, scale etc) with one of those sprays and that normally does the trick.
Hey Krystal! I don't, but I've talked to a few other people since posting this and they had the same suggestion. Do you think spraying down a spatula that I might use for weighing out a reagent with RNAse would do the trick?
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