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I've created stable cell lines that have a depletion of a protein that I study. I would like to survey these cells to look for increases or decreases relative to the wild type or scrambled...
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I need to make a 10 mM Tris solution, pH 7.4, that is RNAse free. I have RNAse-free Tris and RNAse free water to make the 10 mM Tris solution. How's the best way logistically to weigh out the...
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Our lab has Tris-HCl and nuclease free water (commercially purchased), but how do I prepare this together with the nuclease free water to ensure that it doesn't degrade the RNA I am using?
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I am using 2.5 ug DNA:3 uL of Lipofectamine 2000. I am using OMEM (serum-depleted media) for the transfection. I have tried transfecting both adherent cells and cells in culture. I am plating the...
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