What is the maximum allowed concrete temperature during pouring on site?

01 February 2020 10,060 3 View

I need identification key to aquatic fungi ?

can any one provide me akey about aquatic fungi ?

02 March 2019 8,601 2 View

Small particles in my cell culture plates?

Sometimes I see these small particles surrounding my cells in cell culture plates. Are these particles microbial contamination? The first image is a 22RV1 cells used to be clear in the begining...

08 September 2018 1,004 20 View

Is it necessary to dry LiCl in a vacuum oven and dry TFE with CaCl in order to make LiCl/TFE buffer?

I need to make a LiCl/TFE buffer for diluting PPIase substrate (suc-AAPF-pNA) and I was reading the instruction that I have to dry LiCl in vacuum oven for 24 h and dry the TFE with a dry agent...

01 February 2018 6,115 1 View

How to extract Moment outputs in ABAQUS Finite Element Program?

Moment

10 November 2017 8,296 1 View

I need to modeled circular concrete foundation using ABAQUS, which is better and why brick or triangular elements? Are differences in the results?

modelling of turbine foundation

10 November 2017 6,186 4 View

What the correct method to write gene and primers name?

What is the correct method to write genes and primers name?

08 September 2017 821 4 View

What the methodology to extract melanin from fungi?

What the methodology to extract melanin from fungi?

08 September 2017 8,478 0 View

How to extract melanin from fungi?

how to extract melanin from fungi?

08 September 2017 4,327 0 View

In ANN, what is the difference between test data and validation data?

In ANN, what is the difference between test data and validation data? I notice in many training or learning algorithm, the data is often divided into 2 parts, the training set and the test set. My...

07 August 2015 1,593 3 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to remove the residual PDMS on the silicon mold?

Incompletely cross-linked pdms are difficult to remove.

01 March 2021 798 3 View

The reason that the culture of white fungi Contamination with green mold(Trichoderma), the culture autoclave at121 for 15 min. What is it?

Is the period to autoclave not enough? The inoculation in a hood and flame and UV and alcohol.

27 February 2021 9,356 3 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Can 5-FOA Diffuse Through Agar?

I am preparing to do a gene knockout in the filamentous fungi Coccidioides posadasii targeting pyrG/URA3 so that 5-FOA can be used for selection pressure instead of traditional antibiotic...

16 February 2021 169 4 View

Any specific reason, how sclerotium rolfsii can grow out of Petridish?

Is it related to nutrition or any other reason? Any other fungus have similar kind of growth pattern?

09 February 2021 4,357 4 View

Where can I find Plasmopara viticola?

Hello everyone, For my research I want to infect Vitis Vinifera leaves with downy mildew-causing fungus Plasmopara viticola. Does anyone know where I could find or purchase this fungi? I am...

04 February 2021 5,720 3 View

How to store Fusarium oxysporum for long period in simple methods ?

31 January 2021 1,832 17 View

Cutoff for asv reads?

I got my fungi sequences back from Illumina Miseq sequencing and I'm wondering how many reads do you use as a cutoff? ie. do you delete anything with less than 100 reads? I can't seem to find any...

28 January 2021 5,646 4 View