Dear Colleagues, does anyone know the biopharmaceutical classification of Trimebutine maleate?

I want to know the biopharmaceutical class of trimebutine. Thanks in advance!

09 October 2017 1,983 0 View

Does anyone know how long does ssODN stay in iPSCs following transfection?

I was wondering if ssODN stays in the cell as long as a plasmid (e.g. 72 hours). My guess is not, since ssODN is short (e.g. 100bp) and single stranded. I was wondering if someone has attempted to...

09 October 2017 8,370 0 View

How can I prepare a suspension of urease (3.5 kU/L) ?

I have a solid urease, how can I prepare a suspension of this enzyme containing 3.5 kU/L?

09 October 2014 8,998 13 View

How can I prepare a suspension of urease (3.5 kU/L) ?

I have a solid urease, how can I prepare a suspension of this enzyme containing 3.5 kU/L?

06 October 2014 2,919 12 View

How can I put a blunt-sticky ligation of 6.3kb insert into 2.3kb backbone?

Can anyone give some advice on my cloning? This is probably not the situation that you want to have where your insert is almost 3 times larger than your backbone. I tried several times now. I use...

06 July 2014 6,489 15 View

Why doesn't lentivirus infect my cells?

I am making lenti with the lentivector pLVTHM using packaging vectors pCMVD8.91 and pVSVG. We have been successful previously with our labs protocol but lately we seem to be having problems with...

11 December 2013 8,030 10 View

To randomize or not to randomize (cognitive tests in children)

Hello RG community, I was having a discussion with a colleague about whether it is better to randomize or not cognitive tests in children. If we randomize, we can calculate group averages of test...

01 January 1970 2,047 7 View

No title

02 March 2021 3,060 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View