Does anyone know how long does ssODN stay in iPSCs following transfection?

I was wondering if ssODN stays in the cell as long as a plasmid (e.g. 72 hours). My guess is not, since ssODN is short (e.g. 100bp) and single stranded. I was wondering if someone has attempted to...

09 October 2017 8,370 0 View

Whats the best Amaxa program to transfect H9 hESCs with a large 17kb plasmid?

03 April 2016 4,708 0 View

How can I prepare a suspension of urease (3.5 kU/L) ?

I have a solid urease, how can I prepare a suspension of this enzyme containing 3.5 kU/L?

09 October 2014 8,998 13 View

How can I prepare a suspension of urease (3.5 kU/L) ?

I have a solid urease, how can I prepare a suspension of this enzyme containing 3.5 kU/L?

06 October 2014 2,919 12 View

How can I put a blunt-sticky ligation of 6.3kb insert into 2.3kb backbone?

Can anyone give some advice on my cloning? This is probably not the situation that you want to have where your insert is almost 3 times larger than your backbone. I tried several times now. I use...

06 July 2014 6,489 15 View

Why doesn't lentivirus infect my cells?

I am making lenti with the lentivector pLVTHM using packaging vectors pCMVD8.91 and pVSVG. We have been successful previously with our labs protocol but lately we seem to be having problems with...

11 December 2013 8,030 10 View

To randomize or not to randomize (cognitive tests in children)

Hello RG community, I was having a discussion with a colleague about whether it is better to randomize or not cognitive tests in children. If we randomize, we can calculate group averages of test...

01 January 1970 2,047 7 View

Why should we choose deep learning to machine learning? Why is CNN better for image classification? Can I get Typical Answer?

What Characteristics makes CNN work better?

03 March 2021 1,458 4 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

Is there a classification scheme for rock cavities or caverns with respect to their size and shape?

The range of forms and sizes of cavities in rock is very big. Is there a classification scheme for rock cavities regarding their size and shape?

02 March 2021 4,511 3 View

Image Processing and classification using keras/Tensorflow?

Do you have some recommendations approaches on Image processing and classification of plant diseases using TensorFlow/Keras?

02 March 2021 1,493 4 View

Does anyone have kinetic and kinematic dataset for gait classification?

I want to differentiate between normal and abnormal movement. For this reason, I am looking forward to this type of dataset.

01 March 2021 9,485 1 View

Where can find Medical Image Data Sets for Machine learning research project?

I want to work on medical image(disease) detection and classification. Where can I found Image data sets?

24 February 2021 3,130 3 View

How to perform EEG classification of Alzheimer and control groups?

I am doing a project on classification of EEG signals of Alzheimer's disease and control groups. Previously, I extracted the alpha power of the EEG signals and found out the strengths. I want a...

18 February 2021 8,502 2 View

Is it possible to do Classification in Deep learning with feature extraction for limited samples?

Hi, In EEG datasets, I have taken 200 samples X32 channels X 300 trials. For this particular scenario, I like to do binary classification .can you please guide me on whether shall I proceed in...

17 February 2021 5,132 8 View

Using 1D CNN for pixel classification in an image?

I have a 3D Image of size 100x100x100. I have features (feature dimension 25) extracted for each pixel by considering a small pacth around it. So the input to 1D CNN is 100x100x100x25 reshaped to...

17 February 2021 941 4 View

Can you suggest a good domain identification tool?

I am working on a project to identify sensitive information from text and I am looking into the below categories: -adult content -health -politics -crime Can someone suggest an open source tool...

16 February 2021 4,844 4 View