4 Questions 16 Answers 0 Followers
Questions related from Patrick Ovando-Roche
I was wondering if ssODN stays in the cell as long as a plasmid (e.g. 72 hours). My guess is not, since ssODN is short (e.g. 100bp) and single stranded. I was wondering if someone has attempted to...
10 October 2017 8,414 0 View
I was wondering if anyone knows whats the best Amaxa electroporator program to transfect a large 17kb plasmid. I tend to use CB150 which works ok for plasmids up to 12kb. However, I have not...
04 April 2016 4,753 0 View
Can anyone give some advice on my cloning? This is probably not the situation that you want to have where your insert is almost 3 times larger than your backbone. I tried several times now. I use...
07 July 2014 6,543 15 View
I am making lenti with the lentivector pLVTHM using packaging vectors pCMVD8.91 and pVSVG. We have been successful previously with our labs protocol but lately we seem to be having problems with...
12 December 2013 8,071 10 View
