Dear Colleagues, does anyone know the biopharmaceutical classification of Trimebutine maleate?

I want to know the biopharmaceutical class of trimebutine. Thanks in advance!

09 October 2017 1,983 0 View

Whats the best Amaxa program to transfect H9 hESCs with a large 17kb plasmid?

03 April 2016 4,708 0 View

How can I prepare a suspension of urease (3.5 kU/L) ?

I have a solid urease, how can I prepare a suspension of this enzyme containing 3.5 kU/L?

09 October 2014 8,998 13 View

How can I prepare a suspension of urease (3.5 kU/L) ?

I have a solid urease, how can I prepare a suspension of this enzyme containing 3.5 kU/L?

06 October 2014 2,919 12 View

How can I put a blunt-sticky ligation of 6.3kb insert into 2.3kb backbone?

Can anyone give some advice on my cloning? This is probably not the situation that you want to have where your insert is almost 3 times larger than your backbone. I tried several times now. I use...

06 July 2014 6,489 15 View

Why doesn't lentivirus infect my cells?

I am making lenti with the lentivector pLVTHM using packaging vectors pCMVD8.91 and pVSVG. We have been successful previously with our labs protocol but lately we seem to be having problems with...

11 December 2013 8,030 10 View

To randomize or not to randomize (cognitive tests in children)

Hello RG community, I was having a discussion with a colleague about whether it is better to randomize or not cognitive tests in children. If we randomize, we can calculate group averages of test...

01 January 1970 2,047 7 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

How long does it take to get a modeller(for homology modelling) license?

I submitted my e-mail address on modeller website to get a license. The problem is that, the site cannot process my school e-mail (ewhain.net). I submitted my Lab website and school name so that...

25 February 2021 9,054 3 View