Assuming I want to passage my MCF-7 cells only once a week with one or two media changes over the rest of the week, does anybody know what concentration (by trypan blue assessment) should I seed into my next passage?
I am using DMEM with 10% FBS, 1% penstrep, 0.1% antimycobacterium, in a 5% CO2 incubator.
Dear Ralph Lim,
You may want to look at the following sources:
www.atcc.org/products/htb-22?matchtype=p&network=s&device=c&adposition=&keyword=mcf7%20cells&gclid=EAIaIQobChMI3e2g6KSZ9AIVZWxvBB1viQhjEAAYAiAAEgKy-vD_BwE
https://www.sigmaaldrich.com/US/en/product/mm/scc100?gclid=EAIaIQobChMIvZzz-qWZ9AIVMnxvBB1jwAUkEAAYBCAAEgJN5_D_BwE
Hello Ralph Lim
The passaging concentration of MCF-7 cells in T-25 flask should be 0.5 million cells and in T-75 flask should be 1million cells in low glucose Dulbecco’s modified Eagle’s medium (DMEM) containing 10% fetal bovine serum (FBS) supplemented with 2 mM L-glutamine and 1% Penstrep. The culture medium should be changed at least twice a week or as needed according to media pH. Do not allow cell confluency to exceed more than 90% or else cells will decrease proliferation rate. The doubling time of MCF-7 cells is 30-40 hours.
Best.
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