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Questions related from Ralph Lim
I understand that there are differences in ion preference as far as chelation is concerned when comparing EGTA vs EDTA. But is it ok to replace EGTA in my lysis buffer with EDTA since EDTA...
11 June 2024 1,143 1 View
I'm trying to understand the relative sedimentation rates of certain cell types and for this it would be helpful to know their density in terms of mass per volume. For example, PBMCs have a...
23 November 2022 760 0 View
Did a literature search and was surprised to find a large variety of results, but then I realized they may not all have been dissociated and prepped for sorting. So, if I've taken a sample of...
11 August 2022 8,818 0 View
Hi guys, I am looking to optimize viability staining on peripheral blood mononuclear cells (PBMCs) with acridine orange and propidium iodide (AOPI). I will be plotting a titration curve of...
12 April 2022 6,181 0 View
I am in the process of building and testing a microfluidic cell sorter. We have been sorting PBMCs for our experiment due to their frequent use in flow cytometry and general immunology. PBMCs are...
07 December 2021 4,450 1 View
I have some adherent cells in a flask. Early next week, some of those cells will be trypsinized, passaged and seeded in a new flask for future experiments; so far so good. The rest of the cells...
25 November 2021 8,410 5 View
I thawed some MCF-7 cells 3.5 days ago and seeded them into an untreated T75 flask. The medium used was DMEM with 10% FBS, 1% pen/strep, and 0.1% mycozap, all filtered. According to trypan blue...
15 November 2021 1,348 6 View
Assuming I want to passage my MCF-7 cells only once a week with one or two media changes over the rest of the week, does anybody know what concentration (by trypan blue assessment) should I seed...
15 November 2021 7,465 3 View
