Ralph Lim

6 Questions 5 Answers 0 Followers

Questions related from Ralph Lim

What is the diameter of dissociated human cardiomyocytes after sample prep for flow cytometry?

Did a literature search and was surprised to find a large variety of results, but then I realized they may not all have been dissociated and prepped for sorting. So, if I've taken a sample of...

11 August 2022 8,815 0 View

Acridine orange and propidium iodide (AOPI) titration for flow cytometry of peripheral blood mononuclear cells (PBMCs)?

Hi guys, I am looking to optimize viability staining on peripheral blood mononuclear cells (PBMCs) with acridine orange and propidium iodide (AOPI). I will be plotting a titration curve of...

12 April 2022 6,179 0 View

Looking for an adherent cell line to test microfluidic cell sorting with?

I am in the process of building and testing a microfluidic cell sorter. We have been sorting PBMCs for our experiment due to their frequent use in flow cytometry and general immunology. PBMCs are...

07 December 2021 4,447 1 View

How should I store my daily-use adherent cells?

I have some adherent cells in a flask. Early next week, some of those cells will be trypsinized, passaged and seeded in a new flask for future experiments; so far so good. The rest of the cells...

25 November 2021 8,407 5 View

MCF-7 cells not adhering?

I thawed some MCF-7 cells 3.5 days ago and seeded them into an untreated T75 flask. The medium used was DMEM with 10% FBS, 1% pen/strep, and 0.1% mycozap, all filtered. According to trypan blue...

15 November 2021 1,344 6 View

What's a good passaging concentration of MCF-7 cells?

Assuming I want to passage my MCF-7 cells only once a week with one or two media changes over the rest of the week, does anybody know what concentration (by trypan blue assessment) should I seed...

15 November 2021 7,416 3 View