Normally as a result of thermal unfolding the intensity increases and the wavelength of max absorption blue-shifts as Nile Red interacts with the hydrophobic amino-acids. Is it possible that as a result of thermal unfolding of a heme-protein the wavelength blue-shifts with concomitant decrease in intensity? Is it possible that heme Nile Red interaction may be resulting in energy transfer?
Jeffrey R Brender
Heme is known to be a energy transfer acceptor of Nile Red
http://onlinelibrary.wiley.com/doi/10.1111/j.1742-4658.2005.05019.x/full
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Khawar Sohail Siddiqui
Thanks for this reference! However it is not clear if in case of nile-red to heme energy transfer one would expect the Lambda Max to Red-shift with a decrease in intensity or decrease in both lamda max and intensity is possible?
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