Is anyone familiar with protein isolation from Plant root?

Dear Friends, I am naive in fielf of Proteomics, I have been searching a protocol for isolation of protein from plant root and shoots. I want to do Protein estimation from isolated proteins. If...

10 November 2014 1,044 2 View

Drug for snp

How to find a drug which is responsible for SMAD4 gene mutation of pancreatic cancer?

01 February 2014 8,693 2 View

How can I assemble MiSeq reads?

I am new at sequencing platforms.

01 February 2014 9,841 4 View

Can someone help distinguish bwetween contigs, isotigs and singltons?

When I was reading research papers I found that output of blastx file give these type seqence but in my output I only got contigs. By the way I am not doing transcriptome analysis. I am analyzing...

01 February 2014 2,921 3 View

Can someone suggest some literature on Genome Annotation?

Can anyone suggest some good papers related to Annotation. I have got a result from blast2go but don't know how to write it in my paper. Particularly, related to KEGG and Pfam.

01 February 2014 4,757 2 View

What is the maximum read depth in samtools?

I have a sorted.bam file but I want to generate a vcf file using samtools. I worry about how to set the Maximum Read Depth. I have searched on the net but they all are saying different things. As...

01 February 2014 3,152 1 View

Can soemone advise on the use of Genemark output?

I have used genemark tool for prediction of gene.

31 December 2013 5,076 6 View

Anyone know how to solve problem finding protein database for BLASTx ?

I have query file which i got from genemarkhmm tool and which is in nucleotide format. My problem is I want to run BLASTx but It gives me "No alias or index file found for protein database" error....

31 December 2013 2,436 7 View

How to remove duplicates and filtration from the Roche 454 sequence fasta file?

-

31 December 2013 7,473 3 View

How to find SNP and gene by NGS?

I am new in bioinformatics I want to find SNP which are present in S.pneumoniae resistant against ciprofoxcin. What should I do? How to start process?

11 December 2013 7,314 7 View

error in running a model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

error in modelling model in Abaqus

Excessive rotation of nodes in node set ErrNodeExcessRotation-Step1

17 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MGL tools

20 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

i have some problem with chtMultiRegionSimpleFoam

--> FOAM FATAL ERROR: Maximum number of iterations exceeded From function Foam::scalar Foam::species::thermo::T(Foam::scalar, Foam::scalar, Foam::scalar, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar, Foam::scalar) const, Foam::scalar (Foam::species::thermo::*)(Foam::scalar) const) const [with Thermo = Foam::hConstThermo >; Type = Foam::sensibleEnthalpy; Foam::scalar = double; Foam::species::thermo = Foam::species::thermo >, Foam::sensibleEnthalpy>] in file /home/ubuntu/OpenFOAM/OpenFOAM-4.1/src/thermophysicalModels/specie/lnInclude/thermoI.H at line 66. FOAM aborting #0 Foam::error::printStack(Foam::Ostream&) at ??:? #1 Foam::error::abort() at ??:? #2 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::calculate() at ??:? #3 Foam::heRhoThermo >, Foam::sensibleEnthalpy> > > >::correct() at ??:? #4 ? at ??:? #5 __libc_start_main in "/lib/x86_64-linux-gnu/libc.so.6" #6 ? at ??:?

24 March 2021 0 0 View