Drug for snp - FAQS.TIPS

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Is anyone familiar with protein isolation from Plant root?

Dear Friends, I am naive in fielf of Proteomics, I have been searching a protocol for isolation of protein from plant root and shoots. I want to do Protein estimation from isolated proteins. If...

10 November 2014 1,115 2 View

What would be the KEGG equivalent for drug information?

I have pathways from Blast2go tools and want to find my drug related pathways. My genome is resistant to a particular drug. Thanks in advance

01 February 2014 9,936 2 View

How can I assemble MiSeq reads?

I am new at sequencing platforms.

01 February 2014 9,912 4 View

Can someone help distinguish bwetween contigs, isotigs and singltons?

When I was reading research papers I found that output of blastx file give these type seqence but in my output I only got contigs. By the way I am not doing transcriptome analysis. I am analyzing...

01 February 2014 2,986 3 View

What is the maximum read depth in samtools?

I have a sorted.bam file but I want to generate a vcf file using samtools. I worry about how to set the Maximum Read Depth. I have searched on the net but they all are saying different things. As...

01 February 2014 3,222 1 View

Can someone suggest some literature on Genome Annotation?

Can anyone suggest some good papers related to Annotation. I have got a result from blast2go but don't know how to write it in my paper. Particularly, related to KEGG and Pfam.

01 February 2014 4,821 2 View

Anyone know how to solve problem finding protein database for BLASTx ?

I have query file which i got from genemarkhmm tool and which is in nucleotide format. My problem is I want to run BLASTx but It gives me "No alias or index file found for protein database" error....

31 December 2013 2,502 7 View

Can soemone advise on the use of Genemark output?

I have used genemark tool for prediction of gene.

31 December 2013 5,153 6 View

How to remove duplicates and filtration from the Roche 454 sequence fasta file?

31 December 2013 7,552 3 View

How to find SNP and gene by NGS?

I am new in bioinformatics I want to find SNP which are present in S.pneumoniae resistant against ciprofoxcin. What should I do? How to start process?

11 December 2013 7,392 7 View

How to learn more about SPSS and its Application?

I would like to learn more about SPSS and Its application especially in regards to data analysis. Please suggest me how I can learn more about it. Thank you so much.

11 August 2024 9,101 4 View

Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,138 1 View

Baseline drift in HPLC? What causes this?

Hello, Why do i see this baseline drift when i compare my blank (black) to the sample (blue)? Any suggestions as to why this happened? Thank you!

11 August 2024 3,770 4 View

Text-Communication from the M1 Hand Area using BCI—and then there is Elon Musk?

Willett, Shenoy et al. (2021) have developed a brain computer interface (BCI) that used neural signal collected from the hand area of the motor cortex (area M1) of a paralyzed patient. The...

10 August 2024 7,180 0 View

Has anyone applied Python in the field of textile engineering for data analysis, automation, or smart textiles?

I'm currently exploring the application of Python in textile engineering, specifically in areas like data analysis, process automation, and the development of smart textiles. I'm interested in...

10 August 2024 7,429 2 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How to confirm the site-directed mutagenesis result without performing NGS?

I'm cloning a fragment of 3200 nts into plasmid. The cloning was successful, however, 02 amino acids were mutated. Now I want to fix these 02 aa by site-directed mutagenesis technique using...

08 August 2024 4,645 2 View

How are iso-frequency contours plotted?

Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...

07 August 2024 1,937 1 View

How to prepare the nanoparticle treated fungal sample for Environmental SEM analysis?

A fungal strain was treated with nanoparticles. We want to do an environmental SEM analysis. So could anyone share your views on preparing the sample? Thank you.

07 August 2024 5,307 1 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View