Drug for snp - FAQS.TIPS

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Is anyone familiar with protein isolation from Plant root?

Dear Friends, I am naive in fielf of Proteomics, I have been searching a protocol for isolation of protein from plant root and shoots. I want to do Protein estimation from isolated proteins. If...

10 November 2014 1,044 2 View

What would be the KEGG equivalent for drug information?

I have pathways from Blast2go tools and want to find my drug related pathways. My genome is resistant to a particular drug. Thanks in advance

01 February 2014 9,870 2 View

How can I assemble MiSeq reads?

I am new at sequencing platforms.

01 February 2014 9,841 4 View

Can someone help distinguish bwetween contigs, isotigs and singltons?

When I was reading research papers I found that output of blastx file give these type seqence but in my output I only got contigs. By the way I am not doing transcriptome analysis. I am analyzing...

01 February 2014 2,921 3 View

Can someone suggest some literature on Genome Annotation?

Can anyone suggest some good papers related to Annotation. I have got a result from blast2go but don't know how to write it in my paper. Particularly, related to KEGG and Pfam.

01 February 2014 4,757 2 View

What is the maximum read depth in samtools?

I have a sorted.bam file but I want to generate a vcf file using samtools. I worry about how to set the Maximum Read Depth. I have searched on the net but they all are saying different things. As...

01 February 2014 3,152 1 View

Can soemone advise on the use of Genemark output?

I have used genemark tool for prediction of gene.

31 December 2013 5,076 6 View

Anyone know how to solve problem finding protein database for BLASTx ?

I have query file which i got from genemarkhmm tool and which is in nucleotide format. My problem is I want to run BLASTx but It gives me "No alias or index file found for protein database" error....

31 December 2013 2,436 7 View

How to remove duplicates and filtration from the Roche 454 sequence fasta file?

31 December 2013 7,473 3 View

How to find SNP and gene by NGS?

I am new in bioinformatics I want to find SNP which are present in S.pneumoniae resistant against ciprofoxcin. What should I do? How to start process?

11 December 2013 7,314 7 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View