Vishal Desai

12 Questions 20 Answers 0 Followers

Questions related from Vishal Desai

Is anyone familiar with protein isolation from Plant root?

Dear Friends, I am naive in fielf of Proteomics, I have been searching a protocol for isolation of protein from plant root and shoots. I want to do Protein estimation from isolated proteins. If...

11 November 2014 1,095 2 View

What is the maximum read depth in samtools?

I have a sorted.bam file but I want to generate a vcf file using samtools. I worry about how to set the Maximum Read Depth. I have searched on the net but they all are saying different things. As...

02 February 2014 3,196 1 View

Can someone suggest some literature on Genome Annotation?

Can anyone suggest some good papers related to Annotation. I have got a result from blast2go but don't know how to write it in my paper. Particularly, related to KEGG and Pfam.

02 February 2014 4,804 2 View

Drug for snp

How to find a drug which is responsible for SMAD4 gene mutation of pancreatic cancer?

02 February 2014 8,749 2 View

What would be the KEGG equivalent for drug information?

I have pathways from Blast2go tools and want to find my drug related pathways. My genome is resistant to a particular drug. Thanks in advance

02 February 2014 9,915 2 View

Can someone help distinguish bwetween contigs, isotigs and singltons?

When I was reading research papers I found that output of blastx file give these type seqence but in my output I only got contigs. By the way I am not doing transcriptome analysis. I am analyzing...

02 February 2014 2,969 3 View

How can I assemble MiSeq reads?

I am new at sequencing platforms.

02 February 2014 9,882 4 View

How to remove duplicates and filtration from the Roche 454 sequence fasta file?

01 January 2014 7,530 3 View

Anyone know how to solve problem finding protein database for BLASTx ?

I have query file which i got from genemarkhmm tool and which is in nucleotide format. My problem is I want to run BLASTx but It gives me "No alias or index file found for protein database" error....

01 January 2014 2,485 7 View

Can soemone advise on the use of Genemark output?

I have used genemark tool for prediction of gene.

01 January 2014 5,127 6 View

How to find mutation in antibiotic resistent genome?

I have a 454 sequencing of streptococcus pneumoniae resistant to trimethoprim-sulfamethoxazole antibiotic (SRP000185). I am a newbie so I don't know how to initiate? My aim is to find mutation and...

12 December 2013 3,805 5 View

How to find SNP and gene by NGS?

I am new in bioinformatics I want to find SNP which are present in S.pneumoniae resistant against ciprofoxcin. What should I do? How to start process?

12 December 2013 7,366 7 View