Dr. Narendra Kumar Sharma

11 Questions 40 Answers 0 Followers

Questions related from Dr. Narendra Kumar Sharma

Anyone can help with endnote style for shock?

I tried to search output style for shock journal but didnt find. If any one want to share style or information about same style reference of other journal.

11 November 2015 5,089 1 View

Anyone have idea how to find a miRNA target site?

I wants to find miRNA target site by manual or tool available foe access

09 September 2014 1,769 16 View

Can anyone suggest a reliable tool for the design of qRT-PCR primers when used with sybr green?

Can any body suggest or share idea about primer design for qRT-PCR for gene Perilipin 2 step by step for beginner?

05 May 2014 5,465 10 View

How do you make a ROC curve from tabulated data in R?

I want to make an ROC curve from tabulated data using R. Can anyone share the codes or any tutorial for doing this?

03 March 2014 7,194 3 View

Can anyone want to share method for PCA plotting or suggest a free tool for PCA analysis of proteomic data?

Thanks in advance.

02 February 2014 2,716 4 View

Which methods is best for comparing the Plasma proteome between two groups by LC-MS?

I have plasma samples and want to compare two groups to identify differentially expressed proteins by LC-MS. There are several approaches like label free and iTRAQ. I want to know which method is...

02 February 2014 3,364 11 View

Is it possible to identify different oxidized or reduced state of a protein in mixture by mass spectroscopy?

I want to study a signaling molecule which shows reduced or oxidized nature.

02 February 2014 6,973 4 View

Is a cut-off filter suitable to exchange buffer from protein samples?

I experienced, that when I used 3 or 5 kd cut-off filter tubes to exchange buffer for proteomics work, the flow through have some protein content. Theoretically it should not have happened. In...

01 January 2014 7,721 3 View

How can we inhibit a particular signaling pathway?

To silence genes of a particular pathway to study the significance of said pathway.

01 January 2014 9,784 9 View

Is there any free software to make hierarchical clustering of proteins and heat maps with expression patterns?

01 January 2014 1,350 11 View

Is it necessary to acetone precipitate plasma proteins before iTRAQ labelling for mass spec?

As Plasma has high amount of salt and lipids, what is the best methodology for sample preparations before labelling?

01 January 2014 8,744 3 View