What is the difference between Hex Dominant and Cut Cell Meshing methods in CFD?

Though both the methods look similar I believe there may be differences in them. What are the disadvantages of cut-cell meshing method and why is not widely used in research in comparison with...

12 July 2020 5,636 3 View

Is SIMSCALE software a good to go option for visualizing and understanding academic concepts?

On what scale can SIMSCALE simulation software be helpful for students in their engineering course work comprising CFD ( Computational fluid dynamics ), thermal and structural concepts? Can...

29 March 2020 2,952 3 View

What is the correct procedure to balance the e viscous radiator fan. should we balance it with full assembly like fan, drive with visous oil & clutch?

1. How is / should be the balancing of Viscous Fan carried ? Is balancing of Fan blade & Drive separately to meet final assembly specification a correct method of balancing ? or it should be...

10 November 2019 1,782 1 View

How is the processive enzyme which ratchets DNA through a (oxford) nanopore attached the "right way about" to the nanopore?

I am curious about how the ratcheting of a DNA strand through a nanopore works in (oxford) nanopore sequencing. I think that the enzyme motor is first attached to one end of the DNA strand to be...

30 August 2018 7,786 1 View

What are the purification techniques available for water purification other than UV and RO system?

I need low cost water purification technique with readily available material for designing solar water heater cum purifier for rural households.

22 December 2017 864 2 View

Problem with DNA standard optamization?

I am preparing DNA standard curve using gag cloned plasmid by SYBR green assay. I have optimized my primers concentration (250nM) and prepared different DNA concentration based on the copy number....

29 August 2017 1,553 2 View

In scan rate study (sensor), why the diffusion controlled process shows negative intercept and adsorption controlled process shows positive intercept?

Because, I am getting negative intercept for my dopamine sensor (diffusion controlled process). When i am plotting the peak current vs square root of scan rate, i am getting negative intercept....

16 April 2017 2,200 3 View

Is there any way to find if my splice mutation has caused an alternative splice site .?

Although i have the cDNA sequence by RT- PCR..how do i still prove the alternative splicing

10 March 2017 8,262 3 View

Weather 3 wheels or 4 wheels are most suitable for hybrid electric vehicles?

what are the flexibility/importance of wheel selection in hybrid electric vehicle.

25 January 2017 4,768 2 View

GSH determination in cell culture?

I have a protocol that follows DTNB method for tissue and blood samples. I want to adopt this method for cell culture in combination with TCA treatment of cell pellets. Will this work?

04 October 2016 4,672 2 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View