The protein loading dye has SDS, so now the proteins are all linearized and they have the same charge to mass ratio because of which they will be separated based only on the molecular weight.
The dye also has glycerol which being denser will allow the proteins to settle down in the wells.
So I suppose there is no need of the stacking gel.
The stacking gel allows all the proteins to be sorted in order of molecular weight before they enter the separating gel. It also concentrates the proteins into a narrow band. Without it, the bands would be much broader.
The different concentrations of acrylamide and different pH in stacking and resolving gels have been developed for specific reasons (1. the mobility of the proteins moving in the stacking gel is immediately slowed down when they reach the resolving gel. 2. from that point the proteins start to migrate in the resolving gel much slower as very concentrated and sharp protein bands). You can never achieve that effect using just resolving gel.
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