Rini Rahiman

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Questions related from Rini Rahiman

What will happen if we use only resolving gel in SDS PAGE electrophoresis?

The protein loading dye has SDS, so now the proteins are all linearized and they have the same charge to mass ratio because of which they will be separated based only on the molecular weight. The...

06 June 2015 3,258 3 View

Why do we always make proteins negatively charged in a protein gel by using SDS?

Can we make the proteins positively charged to help in its denaturing? If yes, Then using what? and why is negative coating of proteins preferred in lab during SDS PAGE?

06 June 2015 9,114 4 View