What is the minimum number of amino acids in a peptide to get stain with coomassie brilliant blue?

Zymography techniques are based on the digestion of substrate which are included in the sds gel. After digestion, the place around the hydrolytic enzymes are unstained and white. so i am wondering...

11 December 2017 10,011 0 View

Is any article published related to PCR amplification of single (copy) template DNA?

I want to refer the article related to PCR amplification of single copy DNA template. If any published paper available, please upload to me. I am curious to know about that.

11 December 2017 9,186 0 View

What is the frequently used SDS PAGE for protein separation of Eukaryotic cell?

I want to know the best method to separate proteins in Eukaryotic cell for western blot in well established research Lab. Normally SDS PAGE and Gradient SDS PAGE are the two popular methods for...

11 December 2017 8,633 0 View

What is the reason for failure of phytocompound in cancer treatment?

Most of the phytocompounds are shown to be apoptotic, arresting cell cycle in the cancer cell line. Preliminary studies in the cancer cell lines indicates that maximum of the compounds inhibits...

11 December 2017 6,112 0 View

What is the total number of base pair to give signal in real time with sybr green dye?

During real time pcr, the sybr green dye gives signal at a particular cycle in which sufficient amount of dsdna has formed. I just want to know the number of base pair(sufficient amount) to give...

11 December 2017 4,027 0 View

What is the minimum ct value for 130 to 200 bp length product in realtime using sybr green?

I want to know the total minimum bp of dsDNA needed to give the signal in Real time pcr using SYBR green dye. ie 150bp x 10^5 copies = 15 X 10^6 bp of total bp. Kindly share your experience...

10 November 2017 7,023 0 View

What are the common methods frequently used for Characterization and Authentication of Cancer Cell Characterization of Cancer Cell line?

I want to know the methods which are frequently used for Characterization and Authentication of Cancer Cell line in a molecular biology lab cancer drug screening.

10 November 2017 6,049 4 View

How to amplify less copy number gene in Realtime PCR?

I am optimizing real time primers for 10 genes in normal pcr along with GAPDH. Two genes got amplification (GAPDH too) and remaining dosen't. i was using diluted cDNA at the rate of 1:10 as...

10 November 2017 3,132 3 View

Is it possible to detect cell line expressed protein using DAB substrate in westernblot ?

Currently i am expressing a particular MMP in cell line (vero). signelling sequence of mmp was removed. The vector was having CMV promoter and transfected using PEI which was showing more than 50%...

06 July 2017 3,325 0 View

How the Histidine provides positive charge to the protein ?

The pI of Histidine is about 7.5 which is almost equal to the physiological pH. So the total charge of Histidine will be neutral at physiological pH. Then how the His provides positive charge...

04 May 2017 5,899 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View