will the chilled media can kill the cells ?
Zymography techniques are based on the digestion of substrate which are included in the sds gel. After digestion, the place around the hydrolytic enzymes are unstained and white. so i am wondering...
11 December 2017 10,114 0 View
I want to know the best method to separate proteins in Eukaryotic cell for western blot in well established research Lab. Normally SDS PAGE and Gradient SDS PAGE are the two popular methods for...
11 December 2017 8,741 0 View
I want to refer the article related to PCR amplification of single copy DNA template. If any published paper available, please upload to me. I am curious to know about that.
11 December 2017 9,284 0 View
Most of the phytocompounds are shown to be apoptotic, arresting cell cycle in the cancer cell line. Preliminary studies in the cancer cell lines indicates that maximum of the compounds inhibits...
11 December 2017 6,211 0 View
During real time pcr, the sybr green dye gives signal at a particular cycle in which sufficient amount of dsdna has formed. I just want to know the number of base pair(sufficient amount) to give...
11 December 2017 4,141 0 View
I want to know the methods which are frequently used for Characterization and Authentication of Cancer Cell line in a molecular biology lab cancer drug screening.
10 November 2017 6,149 4 View
I want to know the total minimum bp of dsDNA needed to give the signal in Real time pcr using SYBR green dye. ie 150bp x 10^5 copies = 15 X 10^6 bp of total bp. Kindly share your experience...
10 November 2017 7,129 0 View
I am optimizing real time primers for 10 genes in normal pcr along with GAPDH. Two genes got amplification (GAPDH too) and remaining dosen't. i was using diluted cDNA at the rate of 1:10 as...
10 November 2017 3,240 3 View
Currently i am expressing a particular MMP in cell line (vero). signelling sequence of mmp was removed. The vector was having CMV promoter and transfected using PEI which was showing more than 50%...
06 July 2017 3,435 0 View
The pI of Histidine is about 7.5 which is almost equal to the physiological pH. So the total charge of Histidine will be neutral at physiological pH. Then how the His provides positive charge...
04 May 2017 6,029 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
How Social Media Affects Your Mental Health
04 August 2024 6,961 3 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
