Are there any exception like work hardening in metals elastic limit due to dynamic loading?

The samples made out of spring steel are taken from same lot. The specimens were shaped following heating to forging temperature - Forming- Hardening -Tempering methods. After storage of almost 6...

11 February 2021 952 6 View

Regarding to presentation of pairwise comparison of data?

22 January 2021 8,648 1 View

What is the main advantage of using observer based fault tolerant controller(FTC) for sensor fault tolerance?

I am working on FTC for handling sensor faults in the system. I have read about observer-based FTC schemes which use error threshold-based logic between the states and estimated states. If a...

10 January 2021 5,771 2 View

Can a luenberger observer (even a modified version) be effective for a nonlinear affine system?

I want to know whether a luenberger observer or a modified form of it be effective for estimating the states of a system of the following forms: 1. x_dot = f(x,u,d); y = x 2. x_dot = f(x,u); y =...

20 December 2020 1,392 4 View

How much Tensile strength and hardness can be acheived in Aluminium 7050 MMC with B4C reinforcement?

I have conducted research on characterisation of Al7050 + B4C by electromagnetic stir casting. The alloy employed in the process is casted alloy and the Composite too is casted. How much strength...

16 November 2020 9,226 3 View

Why whitefly prefer not to infest Pigeonpea?

Whitefly, Bemisia tabaci is pest of broad host range. In pulse crops, it generally infests greengram, blackgram and cowpea but not Pigeonpea. There might be some biochemical or morphological...

09 November 2020 9,199 3 View

Can u suggest how to identify the matrix material and reinforcement particle distribution in the MMC of scanning electron miroscopy (SEM) images?

How to identify the reinforcement particle behavior in SEM images

09 November 2020 8,664 3 View

Can someone help me in designing off-MPPT mode in a Standalone DC Microgrid?

I am working on voltage control of a Standalone DC Microgrid consisting of Single PV source, a battery and loads. The PV is interfaced through Buck converter to the DC bus, the battery is...

01 October 2020 7,071 2 View

Modeling pure shear loads in Abaqus?

I am trying to simulate a pure shear loading on a cubical block with a single brick element. While I am simulating the other stress components are not getting zeros. Can anyone suggest to me how...

22 September 2020 7,649 3 View

Can anyone suggest any introductory book on tensor analysis and tensor calculus for continuum mechanics?

I have searched google. But it would be more helpful to get suggestions from personal experience.

14 September 2020 5,426 5 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

02 March 2021 2,735 2 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View