I did FTIR of a reactive dye's powder. But I see that the transmittance of 3 peaks went opposite direction of normal peaks and eventually the transmittance percentage became more than 100%. What could be the cause of this?[ Please see that attached figure]
Most probably, this is due to an over-correction from the background that was acquired previously. Or, you forgot to clean (ATR ?) and acquire a background.
Dear friend Shohag Chandra Das
Ah, the perplexing mysteries of FTIR, my friend Shohag Chandra Das! Now, let's dive into this peculiar phenomenon with my fervor.
Firstly, seeing transmittance values exceeding 100% is quite the head-scratcher. Following are points which I will check, if I encounter this kind of results. Based on the visual of your attached figure, there are a few general reasons this might occur:
1. **Data Processing Artifacts:**
- It's possible that during data processing, there was some error or artifact introduced. Check your data processing steps, baseline correction, and any mathematical manipulations that might have led to unintended results.
2. **Saturation:**
- If the peaks are very high, the detector might have saturated. In simple terms, it reached its upper limit and couldn't accurately measure the intensity, leading to distorted results.
3. **Instrument Issues:**
- Check the instrument's health. Misalignments, contamination, or malfunctions can lead to strange results. Ensure your instrument is calibrated and functioning correctly.
4. **Sample Anomalies:**
- The nature of your reactive dye's powder might be unconventional. It could be interacting with the IR radiation in a peculiar way, causing unexpected results. Consider checking the purity of your sample.
5. **Reference Material:**
- Confirm that your reference material (if used) is appropriate and not causing anomalies. The reference material is crucial for accurate FTIR measurements.
6. **Noise or Interference:**
- External factors or noise during the experiment might also contribute. Ensure a controlled environment during your measurements.
Now, I must remind you Shohag Chandra Das, these are speculative suggestions. It's vital to meticulously review your experimental setup, procedures, and the characteristics of your sample. The anomaly might hide in the details.
And remember, while I can offer spirited insights, consulting with experienced colleagues or experts in FTIR analysis would likely shed more light on this intriguing matter. Cheers to the pursuit of knowledge!
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