Please help me in the characterisation of SEM and FESEM pictures. what we should exactly detremines by seeing these pictures
Actually you should know what you want determine. Why you are doing electron microscopy?
If you are looking with your friends to some image in landscape I can promise that everybody will see something else. This is the problem with images and simply eyes as detector. Moreover, SEM images are no light microscopy images. The look often similar to what we see in light microscope, but you have to take into account that you are scanning point by point a sample with an electron beam and you are only counting electrons, photons or anything else. Frankly speaking, you even do not know, whether the beam you are controlling is reaching your sample surface. This is certainly not the case in your example, I only want point out that you need to be damn careful with SEM images or any signal collected since it can become much more complicated than you expect.
Coming back to your question, I only can agree with M. Dellanoy. If you don't say anything about the materials nobody can give you a serious answer. And even if...you don't have to expect that somebody can give you more than a rough speculation. I can only recommend to everyone: If you don't have any idea why you are using a specific technique than don't do it! You are only wasting time, mainly of other people. But maybe your question was not well formulated?
Actually you should know what you want determine. Why you are doing electron microscopy?
If you are looking with your friends to some image in landscape I can promise that everybody will see something else. This is the problem with images and simply eyes as detector. Moreover, SEM images are no light microscopy images. The look often similar to what we see in light microscope, but you have to take into account that you are scanning point by point a sample with an electron beam and you are only counting electrons, photons or anything else. Frankly speaking, you even do not know, whether the beam you are controlling is reaching your sample surface. This is certainly not the case in your example, I only want point out that you need to be damn careful with SEM images or any signal collected since it can become much more complicated than you expect.
Coming back to your question, I only can agree with M. Dellanoy. If you don't say anything about the materials nobody can give you a serious answer. And even if...you don't have to expect that somebody can give you more than a rough speculation. I can only recommend to everyone: If you don't have any idea why you are using a specific technique than don't do it! You are only wasting time, mainly of other people. But maybe your question was not well formulated?
The interpretation of pictures from microscopy is depend on the specimen specification. Sometimes, it is possible to say exactly - what is on a picture, e.g. poxviruses or filoviruses on the negative staining TEM. But in a lot of cases an annotation is very important and it is impossible to say everything without it.
That is a covalent organic polymer i would like to determine the morphology of these samples but i did'nt understood how to determine morphology and the arrangement of molecules.
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