What vector can be used for stable cell line for HEK-293T cell?

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The criterion about maximally localized wannier function?

I heard that as the value of "num_iter(tag in wannier 90)" is higher, spread of Wannier function(=WF) is gradually lower in wannier 90. If so, is this procedure that minimize the spread of WF...

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Seeking Feedback on Methodology for Developing Dynamic, Cost-Aware Resource Allocation Algorithms in Multi-Cloud Environments?

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Wannier90 error: Could not find projections block in wannier90.nnkp?

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The relationship between Partial charge & Lowdin charge in Quantum espresso.?

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24 April 2024 9,487 0 View

How to prevent metal atoms in the active site from forming any bonds with the ligand by using glide in Maestro?

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How to improve the nitrogen use efficiency of rice in your contry?

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What does it mean meta-analysis P-value, I2 ?

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26 March 2024 4,042 4 View

How to cite a paper without the page number information?

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What are the reasons of instability in the process of laser plasma expansion?

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Code for finite element analysis that allows for the specification of some of the mesh node coordinates?

I am looking for a code (open source preferably) for finite element analysis that allows the user to specify some of the node coordinates of the mesh. The code should be able to generate and...

05 February 2024 6,267 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

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How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

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How to use Density Functional Theory to calculate carrier mobilities of solid system?

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Does long-term culture of murine Monocytes result in macrophage differentiation?

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To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

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How can I get my Granzyme B flow cytometry stain to be consistent?

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Best mask design for metal deposition aiming build a Perovskite solar cell device?

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How to culture and maintain Lx-2 cells?

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Transfection in HEK293T cells?

Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...

31 July 2024 9,892 4 View

Angel A. Rivera

Hi,

Yes, both should work.

Lingli Li

Both should work. However, the efficiency would depend on the characteristics of your target genes. Such as the size and level of expression extra

Andrew W. Tai

293T cells are neomycin resistant, so you cannot use the pCI-neo plasmid to generate a stable line.