What causes "bubbles" in DNA gel electrophoresis bands?

Hi all, I just ran a gel electrophoresis that shows a bubble shape in the bands of the ladder. No other row had this shape, and I haven't seen it before. What causes this? Is there anything that...

02 December 2020 1,380 4 View

How to calculate the amount of glucose from the color of Benedict solution?

I'm planning to use Benidict solution to do research on the amount of glucose in candy, but I'm not sure how to calculate the specific amount of sugar from the Benedict solution. Is there a way to...

10 November 2020 1,380 3 View

How to conduct moderation analysis using PROCESS in SPSS with 2 categorical IVs, 1 continuous moderator and 1 continuous DV?

I'm using Hayes' PROCESS macro in SPSS to carry out a moderation analysis. I have two dummy variables (IV1 and IV2), one moderator variable (W) and one DV (Y). As the dialog box does not allow...

10 March 2020 6,652 3 View

What is the best protocol to fix cell in order to visualize the membrane by Scan electron microscopy (SEM)?

Hi, I want to study the membrane using SEM, I am having some problems with cell fixation. I am using PFA and glutaraldehyde , and one lasts step with HDMS ( hexamethyldisilazane). But I think the...

21 November 2019 408 3 View

How is depth information encoded in the frequency of spectral interferograms obtained by Spectral domain optical coherence tomography?

I am trying to understand how the depth information in SD-OCT A-scans is obtained over distances far in excess of the source coherence length. From what I have gathered so far, it seems that the...

10 June 2019 6,783 2 View

Acidification of biochar to lower its pH?

I am trying to test my biochars (charred biomass residues) in ammonia absorption tests. The more acidic biochars (hydrochars) work better, they have a lower pH than the high temperature chars....

11 October 2018 181 7 View

What could be the cause of the double bands showing after running my PCR product on a gel?

After running my PCR on a gel, I got double bands showing for all the positive samples. However, the expected band was brighter than the other band I observed. The cycling conditions were 94 C- 5...

26 March 2018 1,676 3 View

What is wrong with my Western Blot detection?

06 June 2017 4,792 4 View

What software is now being used in place of KC Junior for BioTek platereaders

We used to use KC Junior to run our Elisas and XTT assays as well as our luminometer. Having installed new computers on our plate readers we now are informed that KC Junior is no longer available...

14 April 2016 1,190 3 View

If two variables covary, how do you interpret it?

18 February 2016 9,537 3 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,383 1 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

What could be reason for getting such type of graph for complex impedance in case of polymer blend films?

Its effect appears in real part of ac conductivity also. Can anyone suggest any reason behind it?

26 February 2021 3,487 3 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View