What causes "bubbles" in DNA gel electrophoresis bands?

Hi all, I just ran a gel electrophoresis that shows a bubble shape in the bands of the ladder. No other row had this shape, and I haven't seen it before. What causes this? Is there anything that...

02 December 2020 1,380 4 View

How to calculate the amount of glucose from the color of Benedict solution?

I'm planning to use Benidict solution to do research on the amount of glucose in candy, but I'm not sure how to calculate the specific amount of sugar from the Benedict solution. Is there a way to...

10 November 2020 1,380 3 View

Which is the better procedure to revive pneumococcal strains stored at -80C?

after identifying pneumococcus bacteria, i store it using 30% glycerol at -80 C. One month later i tried to reisolate it. All i have is a sterile blood plate with no growth.

10 March 2020 1,650 2 View

How to conduct moderation analysis using PROCESS in SPSS with 2 categorical IVs, 1 continuous moderator and 1 continuous DV?

I'm using Hayes' PROCESS macro in SPSS to carry out a moderation analysis. I have two dummy variables (IV1 and IV2), one moderator variable (W) and one DV (Y). As the dialog box does not allow...

10 March 2020 6,652 3 View

What is the best protocol to fix cell in order to visualize the membrane by Scan electron microscopy (SEM)?

Hi, I want to study the membrane using SEM, I am having some problems with cell fixation. I am using PFA and glutaraldehyde , and one lasts step with HDMS ( hexamethyldisilazane). But I think the...

21 November 2019 408 3 View

How is depth information encoded in the frequency of spectral interferograms obtained by Spectral domain optical coherence tomography?

I am trying to understand how the depth information in SD-OCT A-scans is obtained over distances far in excess of the source coherence length. From what I have gathered so far, it seems that the...

10 June 2019 6,783 2 View

Acidification of biochar to lower its pH?

I am trying to test my biochars (charred biomass residues) in ammonia absorption tests. The more acidic biochars (hydrochars) work better, they have a lower pH than the high temperature chars....

11 October 2018 181 7 View

Can you identify this insect ?

This insect was found in a rural zone (Ras Elma) in Moulares Gafsa Tunisia plz try to identify this insect at least ordre and family Regards

14 May 2018 4,298 8 View

What could be the cause of the double bands showing after running my PCR product on a gel?

After running my PCR on a gel, I got double bands showing for all the positive samples. However, the expected band was brighter than the other band I observed. The cycling conditions were 94 C- 5...

26 March 2018 1,676 3 View

What is wrong with my Western Blot detection?

06 June 2017 4,792 4 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

Is is possible to pole with corona poling pellets of the sample material instead of thin films?

As it is highly probable that pellets will have a higher degree of air gaps and voids than thin films, is it possible to pole the samples with corona poling nevertheless? Or is there the...

24 February 2021 1,349 1 View

Can anyone help me how to get the similar bands in Actin?

I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...

22 February 2021 6,937 3 View

What to do with absence of fluorescence label in Fucci2a mouse-derived fibroblast culture?

Dear all, I have harvested fibroblast cells from R26p-Fucci2 mouse embryos (mouse line as described in this paper: https://dev.biologists.org/content/140/1/237) and cultured them on plastic,...

17 February 2021 3,368 2 View

Hi, can anybody help me with the suggestions how can I amplify a stress induced gene of 1107 bp?

I am trying to amplify the TGA1 (Transcription factor 1 ) of Arabidopsis ,which is 1107 bp. I have treated the plants with Salycylic solution over night and extracted the RNA and make cDNA. I...

17 February 2021 7,802 2 View

Is it possible to remove the cells from a tissue and keep only the extracellular matrix for proteomics?

Hello! I would like to know if there is a way to flush/remove all the cells out of a tissue and keep only the extracellular matrix, in order to analyse its composition using e.g. proteomics....

14 February 2021 1,602 4 View

Should the AUC in a concentration-time graph of a subcutaneous implant be roughly the same for poeple?

I have 2 concentration time curves of a subcut drug implant. the first group has a peak around the 50 day mark where as the second group does not. Both groups received the same implant by the same...

10 February 2021 2,305 3 View

Meniscus formation matrigel?

Hey guys, I am currently doing tube formation assay on matrigel coated coverslips, and i wonder if there is any possibility to get rid of the meniscus formation? I have only a small part of the...

09 February 2021 6,979 3 View

Why are my western blot bands so distorted?

Hi guys I'm out of ideas here please help! I've never had consistent problem like this doing westerns before and I can't figure it out. The condition used: - First 4 lanes have 50ug protein in...

04 February 2021 2,297 3 View