What causes "bubbles" in DNA gel electrophoresis bands?

Hi all, I just ran a gel electrophoresis that shows a bubble shape in the bands of the ladder. No other row had this shape, and I haven't seen it before. What causes this? Is there anything that...

02 December 2020 1,380 4 View

How to calculate the amount of glucose from the color of Benedict solution?

I'm planning to use Benidict solution to do research on the amount of glucose in candy, but I'm not sure how to calculate the specific amount of sugar from the Benedict solution. Is there a way to...

10 November 2020 1,380 3 View

How to conduct moderation analysis using PROCESS in SPSS with 2 categorical IVs, 1 continuous moderator and 1 continuous DV?

I'm using Hayes' PROCESS macro in SPSS to carry out a moderation analysis. I have two dummy variables (IV1 and IV2), one moderator variable (W) and one DV (Y). As the dialog box does not allow...

10 March 2020 6,652 3 View

What is the best protocol to fix cell in order to visualize the membrane by Scan electron microscopy (SEM)?

Hi, I want to study the membrane using SEM, I am having some problems with cell fixation. I am using PFA and glutaraldehyde , and one lasts step with HDMS ( hexamethyldisilazane). But I think the...

21 November 2019 408 3 View

How is depth information encoded in the frequency of spectral interferograms obtained by Spectral domain optical coherence tomography?

I am trying to understand how the depth information in SD-OCT A-scans is obtained over distances far in excess of the source coherence length. From what I have gathered so far, it seems that the...

10 June 2019 6,783 2 View

Acidification of biochar to lower its pH?

I am trying to test my biochars (charred biomass residues) in ammonia absorption tests. The more acidic biochars (hydrochars) work better, they have a lower pH than the high temperature chars....

11 October 2018 181 7 View

What could be the cause of the double bands showing after running my PCR product on a gel?

After running my PCR on a gel, I got double bands showing for all the positive samples. However, the expected band was brighter than the other band I observed. The cycling conditions were 94 C- 5...

26 March 2018 1,676 3 View

What is wrong with my Western Blot detection?

06 June 2017 4,792 4 View

What software is now being used in place of KC Junior for BioTek platereaders

We used to use KC Junior to run our Elisas and XTT assays as well as our luminometer. Having installed new computers on our plate readers we now are informed that KC Junior is no longer available...

14 April 2016 1,190 3 View

If two variables covary, how do you interpret it?

18 February 2016 9,537 3 View

Looking for a scale to measure attitudes towards violence against women and the factors that impact attitudes in South Africa?

I am interested in doing research on attitudes towards violence against women and the factors that impact attitudes in South Africa? Does anyone know which attitude scale I can use to measure...

06 February 2021 3,076 1 View

How do I access Domestic Violence Attitude Scales or find them for free?

Hi, I'm a postgraduate student currently starting up a research project on attitudes towards domestic violence (DV). The study will aim to compare cultural differences between attitudes and...

31 December 2020 781 3 View

How can i published my full length thesis?

I have done a research paper for the partial fulfillment of my MA program at Bahir dar university "The nexus between ethnic federalism and ethnic conflicts in Ethiopia. the case of Meskan and...

31 December 2020 2,412 3 View

What could be the role of researchers to reduce violence in school?

I want to promote peace and non-violence in school and collaborate for such initiative in future.

30 December 2020 973 3 View

As Europe embraces vaccination, wondering how Africa would accept the idea of Vaccination. Do you think Covid Vaccination would be popular in Nigeria?

Covid 19 Vaccination

25 December 2020 567 16 View

Can I get Sectoral FDI data for Nigeria?

Hello All, I am currently doing my dissertation on the impact of FDI on Nigerian economy on sectoral basis. I have gleaned the databases of all relevant local and international agencies for data...

30 November 2020 6,863 4 View

The attitudes of religious groups towards the relations within a family?

I am looking for the most recent scientific findings concerning attitudes of religious groups towards the relations within a family (equality, subordination, pshysical violence toward wife or...

19 November 2020 6,416 4 View

Hi, I Am Currently Researching On Wind Energy In Nigeria. Pls, Which Software Can Plot This For Me Or How Can I Get The Same Plot For Nigeria?

This is a plot of wind speed in America at 100m and 80m. what software can i use to plot this

17 November 2020 914 8 View

Any ideas that could help me defining the research gap (international business) ?

greetings fellow researchers I'm a phd student in my first year, working on my dissertation "internationalisation in the age of changing workplace" I would like to have different opinion about...

06 October 2020 929 9 View

What are the factors that influence the development of a workplace learning strategy?

Hello, I am presently working on my research proposal for doctoral works with the above in mind. Any proposals comments will be most welcome. The dissertation will be done in Mauritius - a small...

16 July 2020 7,494 3 View