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Questions related from Lydia Ostmo
How soon is too soon to re-preserve HEK293T cells once you've thawed them? We are needing to thaw some cells from the -80 to check for protein expression, but the vials we're thawing are the only...
06 March 2025 7,352 2 View
Hello, I'm facing a conundrum and was hoping to get some advice. We have 12 cryo tubes (all replicates of the same clone) of HEK293T cells preserved in our -80 that have supposedly had a plasmid...
04 March 2025 8,236 1 View
So, this may be a silly question, but what kind of graph is this? (see attached file). It seems to be a combo of a bar graph and a scatter plot? Can these be generated in Excel? If not, how are...
30 October 2023 507 0 View
Hello all, I have recently started working with cytosolic/nuclear fractioning of HEK293T cells. My first few attempts were unsuccessful, in that there seemed to be pretty much equal...
26 June 2023 10,044 0 View
Hello all, first post on ResearchGate! I have been performing immunoprecipitations with FLAG beads (abcam, ab270704), eluting using SDS loading buffer (with DTT) and boiling. The eluted products...
23 May 2023 9,090 5 View
