I am planning to test the effect of endocrine hormones (progesterone, estrogen, and prolactin) on the mammary epithelial cell line. I have already purchased EpH4 and MCF12A. Is it suitable to use these two cells for the experiment of this kind? What kind of cells EpH4-Ev? Do they contain ER receptors on the surface? Even if not estrogen and progesterone are steroid hormones. They can readily diffuse into the cells and activate the respective transcription factors. Is this concept true for this case?
Any suggestion in this regard will be very helpful.