I want to isolate fungi on potato dextrose agar but mostly bacteria and actinomycetes were grown on PD agar.
What type of antibiotics can be used to isolate fungi on growth medium that restricts the growth of bacteria or actinomycetes?
Also suggest to me some antifungals that restrict the growth of fungi so that I can get only bacteria or actinomycetes.
Thanks.
Rose bengal and streptomycin could be used to isolate fungi on Martin medium.
Martin medium: KH2PO4 1g, MgSO4.7H2O .5g, peptone 5g, Glucose 10g, agar 20g, 1% bengal 3.3ml, add water up to 1000ml, then add streptomycin (final concentration is 30ug/ml ) before use.
There are also some antibiotics you can use for isolate fungi on PD agar, such as penicillin, streptomycin and rifampin.
Maybe others can give you good advise on the second question.
It is best to use Gentamicin (500 mcg/ml) and Chromphemnicol (50 mcg/ml) in media to inhibit saprophytic bacteria...they are easily available, cheap and heat-stable, thus resist autoclaving..In order to inhibit fungi in media. e.g. MGIT, Nystatin and Amphotericin b is used
For antibiotics we often use gentamycin but in some cases it cannot completely prevent bacterial and especially actinomycetous growth. Ampicillin and rifampicin usually work better in our tests.For antifungal agent we used fundazol, though higher basidiomycetes are tolerant to this compound.
Hi,
You can use kanamycin, it inhibited bacterial as well as actinomycetes growth on plate. for preventing fungal growth you can add Amphotericin B.
Hi
you can use Amoxycilin and many antibiotic to inhibited bacterial colony and actinomycetes
We mostly use Chloromphenicol in Sabouraud dextrose agar to isolate fungi without bacterial contamination. I guess you could use the same antibiotic in PDA to exclude bacteria.
In our experiment we use chloramphenicol to inhibit bacterial growth and actinomycetes and for saprophyte fungi we use cyclohexamide and they are very effective.
So it is best to use combination of Gentamicin and Chloramphenicol to inhibit bacteria...both are heat stable and cheap..
Ihsan Flayyih Hassan please is there any article about using amoxicillin for fungi isolation
thank you
in our work when isolating fungi from soil, we use Martins medium in which streptomycin or Chloramphenicol is added to inhibit bacteria and also add rose bengal to lowering the growth of fast growing fungi so giving chance for low growing fungi to appear on isolation plates
We used Carboxyline to inhibit bacterial contamination in fungal cultures.
Generally, we used Carboxyline in our lab. Actually, Carboxyline is a versatile scale inhibitor and reduced bacterial proliferation (antibacterial).
Chloramphenicol used to inhibit bacterial growth and cyclohexamide will inhibit the grow of most contaminant fungi
Antibiotics like gentamycin, chloramphenicol and streptomycin can be added to fungal media.
Gentamycin can be supplemented to inhibit bacterial growth in fungal media.
There are many of antibacterial antibiotics which use to prevent the growth of bacteria and isolate fungi on the media, the important one is Chloramphenicol with a concentration of 50 mg/l. It can be used befor the autoclaving.
Also, you can add some other antibiotics such as Gentamicin, Streptomycin sulfate, Tetracyclin, etc... to prevent the bacterial growth in Petri dishes media.
You must know that Gentamicin effects on Gram Negative Bacteria only, but Chloramphenicol is effective against both of Gram Positive and Gram Negative Bacteria, therefore it was prefered to use with fungal culture in order to prevent both types of bacteria.
Chloramphenicol and Cycloheximide
Chloramphenicol
Tetracyclin
streptomycin sulfate
Cefotaxime
Gentamycin
yes, chloramphenicol singly (50mg/l) can suppress bacteria but you could also use combination of gentamicin and pencillin G. Autoclaving temperature denatures Gentamicin and Pencillin, add to the media after autoclaving.
The antibiotics are normally added when culturing fungal isolation sample for the very first time, it is meant to suppress the bacterial growth. 50mg/l of chloramphenicol is good enough alternatively use a combination of gentamicin and pencillin G
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