Which software is better to write systematic review paper?

We have seen some authors use some software to write review paper. As obvious, the study of all published papers concerning our studying field is not possible and these software can help us to...

27 February 2021 6,296 1 View

What is the best internal reference for qRT-PCR assays of HepG2 cell line under the T3 treatment?

I want to treat the HepG2 cells with either T3 or GC1 (TR-beta1 agonist) for 24 hours. I'm not sure about the internal control for qRT-PCR, in order to accurate normalisation of gene...

20 July 2019 5,877 3 View

How can I reference a translated book that I have contributed as part of a team work in order to add to my CV publication list?

I'd like to add a translated text book which its translation has been done by me and other contributers , to my publication list of my CV. I'm not sure about how could I reference that? Shall I...

01 June 2019 1,990 6 View

How to model the Voids in a rock with zero elastic properties in Abaqus ?

Hello, I am trying to build a model that represents a rock with different minerals in Abaqus to recover the elastic properties like the image. My question is how to define the voids as a...

19 May 2019 6,362 7 View

Finite Element simulation on Abaqus ?

Hello, I am trying to simulate a triaxial test on a rock that has different materials properties. My target is to prove that by increasing the number of cells in the model, I will get more...

17 May 2019 9,772 3 View

Is there any tool or survey to investigate students perceptions on the importance of problem-solving skills, this is for students analysis?

is there any tool or survey to investigate students perceptions on the importance of problem-solving skills, this is for students analysis?

14 January 2019 1,362 3 View

Is it better to measure secreted PCSK9 from medium cultured HepG2 cells or doing western blotting?

I want to treat the HepG2 cells with a drug that down regulates the expression of PCSK9. for assay the result, which method is better?

12 January 2018 7,303 2 View

Does anyone have protocol for lentiviral transduction of HepG2 cell lines?

Hi I need to generate TR-beta1 overexpressed HepG2 cell lines. I could not find any protocol for HepG2 transduction by lentiviral system.

30 December 2017 560 2 View

How can I choose the best plasmid vectors for lentivirus production that have my interest gene?

Hi I want to generate TR-beta overexpressed HepG2 stable cell line. For generating a lentiviral vector that have my interest gene insertion (TR-beta), that have puromycin or blasticidin...

26 December 2017 6,465 3 View

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For studying the role of these receptors in my gene of interest regulation in liver I need to have these cell lines

19 September 2017 4,056 1 View

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02 March 2021 3,060 3 View

Is there a method of determining feed digestibility without the need to re-pellet the feed?

I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....

28 February 2021 7,967 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

What is the best surface to culture cells isolated from a skin biopsy?

Hello, recently I am having some difficulties with maintaining the cell culture of primary cells isolated from a skin biopsy (normal and malignant). The coating of culture plates with Matrigel (a...

26 February 2021 6,151 2 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Has anyone experienced changes in growth for cell culture when purchasing media from different companies?

I recently switched the company where we buy our DMEM/F12 and i have noticed a signficant change in growth for my human olfactory neurosphere cell lines

24 February 2021 2,268 3 View

Advice on growing lactobacillus species in the lab?

Hello everyone, For part of my PhD project, I'm hoping to grow some lactobacillus species in the lab so that I can create standard curves for a qPCR quantification of a faecal sample. However, I...

24 February 2021 3,157 6 View

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22 February 2021 9,932 11 View