Reaction condition to convert NH2 to COOH?

I need to couple Dopamine by NH2 end to silica nanoparticles coated by PEG-NH2, but first i need to convert the PEG-NH2 to COOH by using succinic anhydrous ... can you suggest a reaction condition ?

13 February 2021 2,768 4 View

Surface modification of silica nanoparticles with PEG?

I'm trying to coat my silica NP with diamine PEG, so i used GOPTS to add epoxy group on the surface of the NP then i'll add the PEG. I can not found protocol for SiNP coating PEG, most of the...

13 January 2021 4,915 4 View

Silica nanoparticles synthesis using Stober method with size rang 30-50 nm?

I'm preparing silica NP controlling the size by using different concentration of NH4OH. 1- 16.75 ml ethanol. 2-0.5 ml TEOS. 3- NH4OH (0.3- 0.5- 0.75- 1 ml) mixed for 24 hr then evaporate the...

27 November 2020 1,504 13 View

Which neuronal cell line contain D1R?

I'm formulating a drug delivery system that could stimulate Dopamine receptor, For that i need to choose cell line that contain D1R and could be simulated using my delivery system... I have read...

20 November 2020 9,104 2 View

Problems in PCR using Folmer primers, can you help please ?

Hello, I'm using the classic Folmer LCO1490 and HCO2198 primers for a venerid bivalve ADN for two months but the results are still negative, I can't see the bands. 1. I tried to change the...

03 November 2020 9,190 3 View

How to culture prokaryote and eukaryote from the gut of marine organism?

Info regarding the type of media and culturing condition would be really appreciated. Thanks

21 July 2020 6,937 1 View

Calculating sample size for a multivariate regression in GPOWER?

Hello I currently have two dependent variables (with 4 measurements each) and two independent variables My question is how to calculate the sample size using Gpower for multivariate multiple...

21 November 2018 5,719 2 View

CDNA conc. for RT-PCR?

what is the recomended conc. to prepar cDNA to run rt-pcr for brain rat tissue.. i used 50ng/ul some primer gave ct value btw 20-22 but other above 30.. ?

30 October 2017 7,016 3 View

How do i increase the RNA 260/230 ratio ?

i did RNA extraction using differnt protocol triazol and invetrogen RNA mini kit .. the 260/280 ratio was 1.9 to 2.1 but for the 260/230 i got 1.5 to 1.8 in both protocols, should i consider it...

29 October 2017 4,780 4 View

How to choose the best fit evolutionary model based on jmodeltest analysis?

I am working on mtCOI sequences of whiteflies and aiming to do phylogeny based on that aligned sequences. I want to see the best fit of my sequences. However the jmodltest gives different models...

09 May 2017 4,826 7 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to upload pdf of my articles published?

How to upload pdf of my articles published?

03 March 2021 2,183 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 932 1 View

APA 7th In-Text citations with two references with three authors with the same surname, but four different author?

I have an issue with APA 7th in-text citation. II have two papers where the first three authors are the same, and the fourth is different. I am using the Mendeley desktop app, and automatically it...

02 March 2021 790 3 View

How to write a scientific report?

We are analysing scientific reports at University in great detail, so I wanted to find a scientific report about how to write a scientific report. Including detail such as what information to put...

02 March 2021 7,602 4 View

Where can I find life cycle inventory or assessment of non woven materials?

Dear network, I am looking for the LCA or LCI or at least the energy balances of the non woven fabrics process for my master project, in specific for natural fibers, yet I have found...

02 March 2021 9,662 1 View

Would adding books published in Lambert Publishers increase my RG impact?

There are some books published in LAP. Shall I add it to the RG?

02 March 2021 2,753 1 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View