Does anyone know what ovarian cancer subtypes HEY and SKOV3 cancer cell lines are? I looked though the literature and I have found some conflicting descriptions.
Hi,
I´m not sure and I could not provide published data at the moment, but I think
HEY: serous papillary ovarian carcinoma
SKOV: clear cell carcinoma
Try this article:
Shaw TJ, MK Senterman, K Dawson, CA Crane and BC Vanderhyden (2004). Characterization of intra-peritoneal, orthotopic, and metastatic xenograft models of human ovarian cancer. Mol. Therapy 10: 1032-1042.
It identifies the original references for many of the most commonly used ovarian cancer cell lines, and fully characterizes the subtype of ovarian cancer they make when xenografted into nude mice.
HEY cells were originally reported to be derived from a moderately
differentiated papillary cystadenocarcinoma. SKOV3 form clear cell adenocarcinomas.
Hi,
HEY are from intermediately diff. serous adenocarcinoma,
SKOV3 from metastasis, unknown histology , form intermed. diff. tumors in mice
Regards,
Zuzana
These are epithelial ovarian cancer cell lines , various molecules are tested on them for drug treatment
SKOV3 is an ovarian cancer cell line with a p53 mutation at codon 89 (CCC-CC). I have not found the histological type of the tumor, from which the cell line is derived either. According to the type I and type II ovarian cancer classification, this should be a type II tumor.
Dan Cacsire Castillo-Tong
The SKOV3 line has been described as both serous and clear cell. The initial report of the cell line did not describe a subtype and ATCC doesn't know.
It is not a real proof- but data from Broad-CCLE assign following (and many other) mutations to SKOV and Hey pointing to subtypes if we look at subtype specific mutation frequencies (Kinde I et al. Sci Transl Med 2013).
SKOV: ARID1A => clear cell
Hey: KRAS+BRAF => low grade serous
Both cell lines do not show p53 mutations- at least in the CCLE data
SKOV3 is p53 Null based on our Western blot and has Arid1A mutation - more likely endometrioid or clear cell.
HeyA8 : KRAS G12D mutation, TP53 wild-type, most likely a low-grade serous
I found in literature that HEY and SKOV3 are high grade serous sub-type. I know it has been a while since you wrote this post, but did you every determine the sub-type on your own, and if so how?
The molecular profile for HEY suggests a low-grade serous carcinoma or endometrioid carcinoma. However, I recently conducted an immunohistochemical profile of HEY and the cells were WT-1 negative. Most likely endometrioid. However, I wouldn't rule out completely a WT-1 negative low-grade serous carcinoma.
This paper is a comprehensive study for evaluating the cell lines in OC studies using genomic profiling. Based on this, the SKOV3 and HEY are very unlikely to be HGSOC.
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