Does anyone know what ovarian cancer subtypes HEY and SKOV3 cancer cell lines are? I looked though the literature and I have found some conflicting descriptions.
Shaw TJ, MK Senterman, K Dawson, CA Crane and BC Vanderhyden (2004). Characterization of intra-peritoneal, orthotopic, and metastatic xenograft models of human ovarian cancer. Mol. Therapy 10: 1032-1042.
It identifies the original references for many of the most commonly used ovarian cancer cell lines, and fully characterizes the subtype of ovarian cancer they make when xenografted into nude mice.
HEY cells were originally reported to be derived from a moderately
differentiated papillary cystadenocarcinoma. SKOV3 form clear cell adenocarcinomas.
SKOV3 is an ovarian cancer cell line with a p53 mutation at codon 89 (CCC-CC). I have not found the histological type of the tumor, from which the cell line is derived either. According to the type I and type II ovarian cancer classification, this should be a type II tumor.
It is not a real proof- but data from Broad-CCLE assign following (and many other) mutations to SKOV and Hey pointing to subtypes if we look at subtype specific mutation frequencies (Kinde I et al. Sci Transl Med 2013).
SKOV: ARID1A => clear cell
Hey: KRAS+BRAF => low grade serous
Both cell lines do not show p53 mutations- at least in the CCLE data
I found in literature that HEY and SKOV3 are high grade serous sub-type. I know it has been a while since you wrote this post, but did you every determine the sub-type on your own, and if so how?
The molecular profile for HEY suggests a low-grade serous carcinoma or endometrioid carcinoma. However, I recently conducted an immunohistochemical profile of HEY and the cells were WT-1 negative. Most likely endometrioid. However, I wouldn't rule out completely a WT-1 negative low-grade serous carcinoma.