What substitutes should I use in place of Pluronic F-127 in synthesis of Nano Particles by nanoprecipitation method?

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DFT calculation method to enhance the speed of spin polarization?

I attempted calculations for hydrogen adsorption on transition metal-doped g-C3N4. I want to know if it is possible to perform geometry optimization without enabling spin polarization, and then...

19 May 2024 3,318 7 View

How to calculate surface free energy from Neumann's equation of state, where contact angle measurements and liquid interfacial energy is given?

ln[Yl(1+cos theta/2)^2] = -2beta(Ys -Yl)^2 +ln(Ys) A plot of the left side of the equation against the liquid interfacial energy gives parabolic curve, and second order fitting is done.

27 February 2024 1,334 1 View

Recommend a literature and studies about STAD Cooperative Learning Method in teaching Science in Junior High School?

I am teaching science and english in junior high school and I have read several studies about STAD cooperative learning model. However I wanted to have an indepth and comprehensive knowledge on...

28 March 2023 1,973 3 View

What is multimodal in teaching strategy?

Define what is a multimodal and what is the difference between unimodal. and how it is used in the strategy of teaching specifically in teaching science in junior high

04 January 2023 9,534 9 View

How do I calculate gas velocities around a stator blade?

I'm given a study which has given gas velocity on stator pressure side and on suction side to be 300 m/s and 400 m/s and given mach number 0.45 and 0.7. I was wondering how the gas velocity was...

03 April 2022 3,093 0 View

Whynis my visual studio 2022 not able to compile a program?

I am a new student of C++ but my visual studio doesnt extract we'll. Do I mids out something during downloading. Three times it downloaded to 100%

03 January 2022 2,868 3 View

What is the influence of the mold release agent on sound absorption properties of polyurethane foam ?

I'm an intern in a big company which makes flexible polyurehane foam for the automotive sector and I'm working on sound absorption properties of PU foam. I'm not chemist so I'm trying to improve...

23 September 2020 3,952 4 View

Problems faced while simulating a dynamic load on pile in Plaxis 2D. Can anyone help?

Hi. I am trying to simulate a rapid load test in Plaxis to determine the load transfer mechanism. The load is inputted as a line load and the dynamic multiplier is defined as a table with 1 at the...

22 July 2020 4,968 1 View

Research on RuangRupa?

The nomination of the Jakarta-based artist group as curator for the 15th Documenta brought worldwide attention to them. A couple of writers have written texts about them, some of the more well...

18 May 2020 6,308 0 View

How to analysis FTIR data?

Hi, everyone, I’m utilizing the FTIR to analysis microplastics. However, I cannot find the C-C bond peak or C-H bond peak in the result(black one). The result was shown in the below. As you can...

02 January 2020 6,476 3 View

How to prevent clots from falling while doing a clot retraction assay with PRP?

Hey everyone, so I am trying to perfect my procedure for the clot retraction analysis and I am struggling to keep the clot on the meniscus line. I am treating my cuvettes with pluronic to prevent...

21 April 2024 3,271 1 View

Pluronic molecules run for Gaussian software? Possible?

higher molecular weight is pluronic

06 November 2023 5,490 0 View

Effect of (Kolliphor) Poloxamer 188 on CHO cells?

In my experiment, I utilized a combination of Poloxamer 188 (Kolliphor) and Gibco Anticlumping agent in Dynamis media at a concentration of 0.1% per liter for CHO S cell culture. Surprisingly, I...

07 June 2023 7,493 1 View

How to prevent sticking of single cancer cells to PDMS microfluidic chip?

I'm doing a flow experiment, flowing single MCF7 cells through PDMS microfluidic chips (connected to a glass microscope slide). Currently, I'm using a Pluronic F127 (0.1 wt%) coating. However,...

21 May 2023 9,628 3 View

Issues with Pluronic F127 polymerisation - Reasons for non-polymerisation?

Hi, We've been having issues with a loss of polymerisation of Pluronic F127. Usually 10% (w/v) solutions prepared in 18.2MOhm water polymerise after preparation at 40°C with continuous stirring,...

07 May 2023 7,288 0 View

Why is cell clumping observed in the CHO-S cell line despite the presence of an anticlumping agent?

What is the reason for cell clumping in the CHO-S cell line despite the presence of an anticlumping agent (1%/L) in Dynamis medium, when the cell density reaches above 3x10^6 cells/mL? Is the...

03 May 2023 7,484 3 View

Pluronic F127 Critical micelle concentration CMC at various temperatures?

Pluronic F127 Critical micelle concentration CMC at various temperatures

20 March 2023 4,138 0 View

Is possible to explain to me the details of the method of preparation of Poloxamer gel?

Hi dear Amir Fakhari I am Reyhaneh Sam, Ph.D. student of Pharmaceutics at Kerman university of medical sciences. I need to know how can I use Poloxamer 407 powder from Sigma Aldrich to see its...

28 January 2023 7,065 1 View

Pluronic F-127 precoating for spheroid formation?

Hi, I was trying to generate spheroids from A375 cells by Pluronic F-127 precoating method. I used 1% F-127 in PBS to cover 12-well plate wells for 1 hour at room temperature, then rinsed the...

11 August 2022 982 1 View

How can I determine the concentration of Pluronics (L64 and L62) in aqueous media by UV-Vis method, viscosity or DLS?

L64 triblock copolymer (Pluronic L64: EO13PO30EO13) and L62 (Polyoxyethylene-polyoxypropylene block copolymer;Poloxamer 182) Thanks a lot!

05 April 2022 7,266 4 View