I'm trying to isolate certain biotinylated cell surface proteins from total cell lysis after proximity labeling. I used RayBio Streptavidin Magnetic Beads but it didn't work well. Tons of biotinylated proteins existed in the flow through. Anybody has used this beads before?
And what's the difference between Dynabeads Streptavidin Magnetic Beads and Pierce™ Streptavidin Magnetic Beads?
Thank you!
Hi,
In terms of the Dynabeads, feel free to reach out at [email protected] - see if I can be of help.
kind regards
ketil
It might be a problem of optimization: have you tried to increase the amount of beads, to see if you can increase the yield of pulled-down biotinylated proteins?
For my proximity labeling experiments, i used the Pierce streptavidin magnetic beads, with good results. But I think there are several good options out there.
Good luck!
Another factor that might reduce the efficiency of streptavidin pull down is actually the amount of biotin you are using for the biotinylation reaction: if it is too much in excess, or if the non reacted biotin is not properly washed away, that could outcompete your actually biotinylated proteins for binding to the beads.
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