Yu Lun

5 Questions 6 Answers 0 Followers

Questions related from Yu Lun

What protein about 100kDa is tyrosine phospholated when challenge T cell with anti CD3?

Hi, I uesd purified mouse CD3 T cell from spleen and stimulated these cells with anti mouse CD3 for different time. At least I can say two proteins are activated (black and red arrow). For the...

12 November 2020 7,337 3 View

Why cultured BMDCs attach to the T75 Flask?

Hi, I used GM-CSF to culture BMDCs but got some problems. On day 1, I seeded the cells in 10mm plate (106/ml) in R10+10ng/ml murine GM-CSF. Medium was changed on day 3 and day 5. On day 6,...

10 September 2020 4,726 1 View

What's wrong with my BMDC culture and typing?

Hi, I am culturing BMDC using B6 mice. After 1 week I harvested the cells and stained with CD11b and CD11c (figure 1). But the double positive cell percentage is only about 40%. Is it...

20 March 2020 4,569 3 View

What's the difference between anti-P-src(Tyr416) and (Tyr 418)?

Hi, I am looking for an anti-P-src antibody which interact with human CD44. Tyr 418 was used in the literature but only Tyr 416 on Cellsignaling website. Are they the same?

17 February 2020 6,303 4 View

What streptavidin magnetic beads are you using?

I'm trying to isolate certain biotinylated cell surface proteins from total cell lysis after proximity labeling. I used RayBio Streptavidin Magnetic Beads but it didn't work well. Tons of...

15 August 2019 1,347 4 View