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Hi, I uesd purified mouse CD3 T cell from spleen and stimulated these cells with anti mouse CD3 for different time. At least I can say two proteins are activated (black and red arrow). For the...
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Hi, I used GM-CSF to culture BMDCs but got some problems. On day 1, I seeded the cells in 10mm plate (106/ml) in R10+10ng/ml murine GM-CSF. Medium was changed on day 3 and day 5. On day 6,...
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Hi, I am culturing BMDC using B6 mice. After 1 week I harvested the cells and stained with CD11b and CD11c (figure 1). But the double positive cell percentage is only about 40%. Is it...
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