When solubilizing inclusion bodies, high concentration of solubilizing agents (like urea, SDS, Guanidine hydrochloride...) are required, which leads to high costs. What strategies can be used to recover and reuse these solubilizing agents in processes for the recovery of proteins from inclusion bodies?
Graciela: When consider costs, did you also calculate how much value your time is worthy? So, re-calculate your cost. Technically, one needs to re-crystallize and remove contaminating proteins in the extraction waste to recover clean SDS, GnHCl, etc. Good luck.
I would not recommend re-using GnHCl and SDS that has once been used for solubilizing your inclusion body protein fraction. Why would you want to cross contaminate your next batch? Because till date, laboratory scale clean up of used reagents is never recommended because of very obvious reasons. I fully agree with what Ke-Wei Zhao points out in the previous response. Look at your cost evaluation in a different manner. By not messing up your next batch of proteins with a ''lab-grade attempted clean up'' of reagents, you are actually saving time, money and effort.
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