I am working with seaweeds. I am using using ethanol as my extraction solvent based on preliminary experiment and using methanol to dissolve my DPPH, should I expect any difference than, when I use ethanol?
Dear Patrick A. Blamo Jr
Since your extract is ethanolic, the DPPH must dissolved in ethanol rather than methanol. Otherwise, it may expect a difference in the reading of the absorbance. I recommend the use of ethanol as a solvent for DPPH.
All the best
I recommend that methanol the best solvent for DPPH desolving for antioxidant assay.
Dear Patrick A. Blamo Jr
Since your extract is ethanolic, the DPPH must dissolved in ethanol rather than methanol. Otherwise, it may expect a difference in the reading of the absorbance. I recommend the use of ethanol as a solvent for DPPH.
All the best
Dear all, DPPH stands for 2,2-diphenyl-1-picrylhydrazyl, It measures the capacity of an antioxydant (AH, generaly a phlenolic compound) to reduce the radical DPPH° (2,2-diphenyl-1-picrylhydrazyl) by hydrogen transfert. Le DPPH°, initially violet, tiens to DPPH-H, yellow pale.
Reduction of DPPH° is easily measured by spectrophotométry at 515 nm (λmax DPPH°).
If it is to be working with referenced basis, methanol is to be used. If you have time and curiousity try both solvents. My Regards
Dear all, the following RG free publication shows the use and comparaison of both solvents. Table 3 sumerizes the comparaison results. My Regards
https://www.researchgate.net/publication/320221956_Antioxidant_antimicrobial_and_antiproliferative_activities_of_peel_and_pulp_extracts_of_red_and_white_varieties_of_Ipomoea_batatas_L_Lam
Dear all, the attached file is a systematic study to elucidate the effect of the alkyl effect of alcoholic solvents. My Regards
Dear Patrick;
I completely agree with Yasser Fakri Mustafa, DPPH is also soluble in ethanol, so it is preferable to use the same solvent.
With my best regards
Since your extraction solvent is ethanol, i will advice you dissolve or prepared your DPPH solution with ethanol rather than methanol so that both the reagent and the sample will have the same polarity.
Patrick A. Blamo Jr It would be better to use same solvent for both extract and DPPH, because you need to use same control blank. if you have dissolved DPPH using methanol, you may dry your extracts then dissolve it in methanol.
i will advice you dissolve or prepared your DPPH solution with ethanol rather than methanol so that both the reagent and the sample will have the same polarity.
Good luck
ethanol is more recommended
but, I've seen the use of both. Generally, Both are acceptable
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