Hi,
For GUS fluorometry protocol, I need to prepare 4-MU for standard curve.
For the 4-MU standard, I will prepare 10ml of 1mM stock first, then dilute to 10ml of 1uM stock to be stored in dark at 4C. 4-MU working solution will be prepared fresh every time by diluting to 0-200nM for standard curve using 0.2M sodium carbonate.
1) When preparing 1mM 4-MU, should I dissolve 4-MU in water or DMSO or 0.2M sodium carbonate? Can I store this in dark at 4C or -20C?
2) What about 1uM stock? should I dissolve 4-MU in water or DMSO or 0.2M sodium carbonate? Can I store this in dark at 4C or -20C?
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