What should I do about overrepresented Clontech SMART CDS Primer II A sequences in fastq files?

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How much amount of DNA (ligation mix) should I use for transformation?

Hi Fellow Scientists! I am trying to clone my 300 bp insert into a 6 kb vector. I run a few different ligation reactions using (1:3 vector: insert molar ratios) 10, 20,30, and 40 ng/ul vector...

15 February 2021 9,563 6 View

How post-hoc test should I perform for two way repeated measures ANOVA for 3 groups?

Hello everyone, I have 3 groups and 4 measurements so, I performed two-way repeated measures ANOVA. How can I detect the difference (which groups and which measurements) when there is significant...

27 October 2020 4,503 3 View

How do We Maintain Appropriate Cell microenvironment with 3D Cell Culture Method?

3D Cell Culture is newly optimized and promising discovery in cell organization research and other researches that needs evindences in cell culture (in vitro) level. Are 3D Cell Cultures mimic...

23 September 2020 7,109 4 View

How do We Clarify the Effects of Drugs on DNA Replication?

DNA Replication and its components are common targets in discovering new drugs as antimicrobial therapeutic agents especially while dealing with antibiotic-resistant species and other diseases...

21 September 2020 6,172 4 View

Can you give information about how to calculate the molarity of MEA?

Hi, I am trying to produce CZTS films by sol-gel spin technique. Can you give information about how to calculate the molarity of MEA? Did you calculate the MEA molarity separately for each metal...

11 July 2020 3,415 1 View

Do weak contribution equations within a module use the module's boundary condition in COMSOL?

I am trying to solve a partial wave equation for scattered quasi-TE waves. I found the COMSOL built-in Helmholtz equation module very close to my problem. The bottleneck was that, I couldn't...

03 June 2020 8,937 1 View

How Can I find a reverse primer with good rating, if I need to amplify entire gene?

I need to amplify the KCNA1 gene. I could not design the reverse primer with GC content ~50%, G/C content between 40-60%, rating 100, and no secondary structure. Any help much appreciated!

25 May 2020 7,276 9 View

Is it possible to have more elements than nodes in mesh analysis of ansys?

During Centrifugal Pump Simulation by using ANSYS, I obtained more elements than nodes. Is this possible or the number of nodes should always be greater than number of elements?

04 May 2020 4,700 11 View

How should I compare pre test and post test of three groups?

Hi everyone, I have 2 treatment groups and 1 control group. I measured some physical parameters as a pre-test and post-test. Should I compare 3 groups pre-test and post-test separately by using...

23 March 2020 8,599 8 View

Plasmonics as a collective oscillation of holes instead of electrons?

In metals, the outermost conduction electrons (electron plasma) interact with incident photons and according to the classical Drude model, they may resonate and build up longitudinal plasmonic...

17 January 2020 1,559 7 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

Why is the efficiency of viral mRNA electroporation in PBMC low?

Hello, I have been struggling with the electroporation of PBMC with HIV-I mRNA. Although the quality of the RNA generated is good, and the handling of the cells in meticulous, the frequency of...

27 February 2021 9,233 3 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View

What is the best concentration of cDNA to be used for qPCR?

I have synthesised cDNA from 500ng/ul of RNA. The concentration of the cDNA Is about 5000ng/ul. Should I dilute my cDNA or just use it as it is for my qPCR? I usually use 2ul of cDNA for a 20ul...

24 February 2021 8,618 3 View