DNA Replication and its components are common targets in discovering new drugs as antimicrobial therapeutic agents especially while dealing with antibiotic-resistant species and other diseases that are related with the disfunction in DNA Replication. Are there any methods or interventions to identify changes in DNA Replication Complex as a result of drug exposure ? Are there isolated total DNA Replication Complex to see consequences of interaction of drugs with the its components in big picture?
The methods to identify effects of replication-targetting drugs are molecular and structural ones. Analyses of crystallized enzymes complexes and DNA-enzyme complexes with/without drugs at high resolution reveal evidences.
The methods employed in this study may give you a good idea:
Wohlkonig et al., 2010. Structural basis of quinolone inhibition of type IIA topoisomerases and target-mediated resistance. Nat Struct Mol Biol.
https://doi.org/10.1038/nsmb.1892
- Badges
- Science topic
- Similar topics
- Chemistry
- Pharmacology
- Pharmaceuticals
- Drugs