What should be the percentage of viable cells in a particular tissue to carry out the Comet assay?

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Milad Esmaeilbeigi

due to in comet assay we need to cells, from the minimum amount of tissue can obtain a lot of cells.

Nilufer Cinkilic Aydemir

Minimum number of viable cells should be 85 % percent.

Yao Akpamagbo

I don't have an answer to this, but how did you determine that you had 70% viable cells in your tissue?